We narrowed to 4,857 results for: Mela

1. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUASp YFP Ras85D CA

   Plasmid

   #52247

   Purpose

   Drosophila expression of YFP-Ras85D constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CMV-SpyCas9

   Plasmid

   #113034

   Purpose

   AAV vector for expression of SpyCas9 in mammalian cells

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCFD5-U6-3-t-attP40

   Plasmid

   #133561

   Purpose

   gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   attP40 region guide RNAs
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Syn-AlstR-IRES2-EGFP

   Plasmid

   #14895

   Depositor

   Edward Callaway

   Article

   Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

   Insert

   AlstR (AstA-R1 Fly)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 11, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 908H

   Plasmid

   #160240

   Purpose

   TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Tet Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSFDuet-1 IntS8 AA 1-308

   Plasmid

   #196905

   Purpose

   Expresses His-tagged Drosophila IntS8 amino acids 1-308

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 8 (IntS8 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217513

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-NLS-AcrIIA4

   Plasmid

   #113038

   Purpose

   AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199195

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAFW-Ago1

    Plasmid

    #50553

    Purpose

    Expression of FLAG tagged Ago1 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

    Insert

    Argonaute1 (AGO1 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-ERBB4 (kinase dead)

    Plasmid

    #29533

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Promoter

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pUASp YFP Ras85D WT

    Plasmid

    #52242

    Purpose

    Drosophila expression of YFP-Ras85D

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. FLAG-Med29 pBacPAK8

    Plasmid

    #15369

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    Med29 (MED29 Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pGC(2.1)-U6.3

    Plasmid

    #170516

    Purpose

    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(2.1)-pU6.3
    

    Use

    Pcr template vector for amplifying grnacore(2.1)-…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabePuro-DNAJA4CDS

    Plasmid

    #43799

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    DNAJA4 CDS (DNAJA4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

17. TOPO tTR-KRAB-rtTA3-Luc

    Plasmid

    #68449

    Purpose

    TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    

    Use

    Luciferase; Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDF1-ERBB4 (kinase dead)

    Plasmid

    #29544

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Promoter

    Available Since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

19. YFP Rab4DN

    Plasmid

    #99019

    Purpose

    Localization and visualization of the dominant negative form of Rab4

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    Rab4 DN (Rab4 Fly, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    S22N Dominant negative

    Promoter

    CMV

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASp YFP Ras85D DN

    Plasmid

    #52246

    Purpose

    Drosophila expression of YFP-Ras85D dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S17N; DN mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pDsred monomer C1- MLPH

    Plasmid

    #89236

    Purpose

    Expression of Dsred-tagged human Melanophilin in mammalian cells

    Depositor

    William Gahl

    Article

    Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

    Insert

    Human Melanophilin (MLPH Human)
    

    Use

    Tags

    Dsred monomer

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-MED29

    Plasmid

    #15409

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED29 (MED29 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3 hML23Cm6

    Plasmid

    #109368

    Purpose

    Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    human Melanopsin-mGluR6 chimera (OPN4, GRM6 Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. K401-Micro

    Plasmid

    #122485

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Matt Thomson

    Article

    Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

    Insert

    His tag MBP Kinesin 1-401 SspB micro
    

    Use

    Tags

    H6-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-401

    Promoter

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. R26TV LSL-TRL

    Plasmid

    #68476

    Purpose

    Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    

    Use

    Cre/Lox, Luciferase, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti6/V5-DEST-UCHL5

    Plasmid

    #31219

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    UCHL5 (UCHL5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

28. OA-1045C

    Plasmid

    #125005

    Purpose

    Expresses gRNAs targeting hid, eve, and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    eve gRNA (eve Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti6/V5-DEST-NDC80

    Plasmid

    #31215

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NDC80 (NDC80 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pUASTattB_NSnoFbox-vhhGFP4

    Plasmid

    #35578

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSnoFbox-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    F box domain of slmb deleted.

    Promoter

    Available Since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

    Plasmid

    #148839

    Purpose

    Mammalian Expression of DmBam_13-36

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    DmBam_13-36 (bam Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Use

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

34. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RCAS(A) dRhoA V14 (CT#846)

    Plasmid

    #13949

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    RhoA (Rho1 Fly)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    G14V mutation

    Promoter

    Available Since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

36. pUAST-SIK3 T196A

    Plasmid

    #52974

    Purpose

    insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    T196A LKB1 phosphorylation defective mutant

    Promoter

    Hsp70

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pUAST-SIK3 K70M

    Plasmid

    #52973

    Purpose

    insect expression of SIK3 K70M (kinase dead mutant)

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    K70M kinase dead mutant

    Promoter

    Hsp70

    Available Since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pLNCX2-Orai1

    Plasmid

    #89818

    Purpose

    Express Orai1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FLAG-MED6

    Plasmid

    #15411

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED6 (Med6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

40. Ths pUAST-HA

    Plasmid

    #69769

    Purpose

    Thisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    Klingseisen et al Development. 2009 Jul;136(14):2393-402.

    Insert

    Thisbe (FGF8like-1)
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA-Tag

    Expression

    Insect

    Mutation

    Encodes amino acids 1-739 of Thisbe (NP_610701.2)…

    Promoter

    hsp70 promoter

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only