We narrowed to 1,621 results for: sp1

1. LT3GEPIR-ARshRNA3

   Plasmid

   #220563

   Purpose

   To inducibly knockdown AR expression

   Depositor

   Sean Lee

   Article

   Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

   Insert

   AR shRNA3 (AR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LT3GEPIR-ARshRNA4

   Plasmid

   #220564

   Purpose

   To inducibly knockdown AR expression

   Depositor

   Sean Lee

   Article

   Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

   Insert

   AR shRNA4 (AR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

   Plasmid

   #136656

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:MTH17CL396 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and hU6

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6_sgRNA_CAG_hSt1Cas9_TH1477

   Plasmid

   #136655

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:TH1477 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and hU6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6_sgRNA_CAG_hSt1Cas9_LMG18311

   Plasmid

   #136653

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:LMG18311 chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and hU6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet151D-TOPO-BROX 1-411; K141E

   Plasmid

   #89865

   Purpose

   Expresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   BROX (BROX Human)
   

   Use

   Tags

   His Tag, V5 tag

   Expression

   Bacterial

   Mutation

   mutated lysine 141 to glutamic acid

   Promoter

   T7

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pet151D-TOPO-BROX 1-411; L212D

   Plasmid

   #89864

   Purpose

   Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   BROX (BROX Human)
   

   Use

   Tags

   His Tag, V5 tag

   Expression

   Bacterial

   Mutation

   mutated leucine 212 to aspartic acid

   Promoter

   T7

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

   Plasmid

   #59827

   Purpose

   Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV-AR

   Plasmid

   #213819

   Purpose

   Lenti-viral expression of human androgen receptor wild-type (WT).

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR (human androgen receptor) (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag (N terminal on backbone)

   Expression

   Mammalian

   Mutation

   No, it is AR wild-type.

   Promoter

   EF-1a

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-mTurquoise2-Caspase-1-mNeonGreen

    Plasmid

    #166784

    Purpose

    FRET analysis (pro caspase-1 cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-AR V7

    Plasmid

    #86856

    Purpose

    Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

    Depositor

    Marco Marcelli, Michael Mancini

    Article

    Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Alternative splice variant 7 (alteration/deletion…

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pinducer 20 DN-KASH

    Plasmid

    #125554

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    tetracycline responsive element

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-AR C619Y

    Plasmid

    #86429

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with point mutation C619Y

    Depositor

    Michael Mancini

    Article

    Nazareth et al Mol Endocrinol. 1999 Dec;13(12):2065-75.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C619Y

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL hygro Flag HA TRAP220 wt

    Plasmid

    #17433

    Purpose

    Retroviral expression of Med1/TRAP220 in mammalian cells

    Depositor

    Kai Ge

    Article

    Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

    Insert

    TRAP220 (MED1 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-HA-AR-DBD

    Plasmid

    #171238

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-HA-AR-NTD

    Plasmid

    #171235

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-AR-LBD

    Plasmid

    #171239

    Purpose

    Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-AR Q0

    Plasmid

    #86427

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the poly-glutamine expansion

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge