We narrowed to 7,700 results for: 104

1. pREF-GFP kan

   Plasmid

   #122061

   Purpose

   reporter plasmid for termination and terminator override to express GFP. Marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Inserts

   intergenic terminator from yeast IMD2
   green fluorescent protein
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL1 comes in pYES2

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mOtop3_pcDNA3

   Plasmid

   #114679

   Purpose

   This construct was used to express mouse Otop3 in HEK-293 cells

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 3 (Otop3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMK375 (Hygro-P2A-Stag-3FLAG)

   Plasmid

   #121186

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-Stag-3FLAG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-Flag-YAP-S94/F95A

   Plasmid

   #33057

   Depositor

   Kunliang Guan

   Article

   Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S94A, F95A

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

5. FLAG-ZnF/HIT1

   Plasmid

   #15332

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

   Insert

   zinc finger, HIT domain containing 1 (ZNHIT1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA4/TO/myc-His-A-YAP2

   Plasmid

   #19060

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Jul 17. ():.

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

7. AL13Rb-GAPC2

   Plasmid

   #161009

   Purpose

   PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

   Depositor

   Krishna Niyogi

   Article

   Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

   Insert

   PP7 and H2B-mScarlet
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GST-N1IC

   Plasmid

   #47612

   Purpose

   bacterial expression of GST-myc-Notch1 IC

   Depositor

   Urban Lendahl

   Article

   Beatus et al Development. 1999 Sep;126(17):3925-35.

   Insert

   Notch1 intracellular domain (Notch1 Mouse)
   

   Use

   Tags

   GST and myc

   Expression

   Bacterial

   Mutation

   contains amino acids 1753-2531

   Promoter

   tac

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

9. PB-Tal1

   Plasmid

   #60670

   Purpose

   PiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Tal1 (Tal1 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tdKatushka2-VSVG-16

    Plasmid

    #56052

    Purpose

    Localization: Golgi/ER Transport, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    VSVG
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pcDNA5-hygro-eGFP-HEC1

    Plasmid

    #114027

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJM126

    Plasmid

    #49339

    Purpose

    bacterial expression of RFA1, RFA2, and RFA3

    Depositor

    Steven Brill

    Article

    He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

    Inserts

    RFA3 (RFA3 Budding Yeast)
    RFA2 (RFA2 Budding Yeast)
    RFA1 (RFA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAcGFP1-N1-RvLEAM

    Plasmid

    #90035

    Purpose

    To express GFP-tagged RvLEAM in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #164046

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    mTagBFG-TriTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDS272

    Plasmid

    #34975

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTW102

    Plasmid

    #115945

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 DDd-L7Ae
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFRT/TO/GFP-SCAF4 (CRISPR_resistant)

    Plasmid

    #122471

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF4 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 (SCAF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-puro-eGFP-HEC1-9A

    Plasmid

    #114023

    Purpose

    Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 9A

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK407 (BSD-P2A-mAID-Nluc)

    Plasmid

    #121197

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID-Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pChlr-8CQ

    Plasmid

    #52171

    Purpose

    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed 12N to 12C in module 8

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

21. PB-Pitx2

    Plasmid

    #60668

    Purpose

    PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Pitx2 (Pitx2 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PAXBP1_pCSdest

    Plasmid

    #53834

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PAXBP1 (PAXBP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

23. mEos2-ER-5

    Plasmid

    #57373

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    ER (CALR Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pFRT/TO/FLAGHA-SCAF8

    Plasmid

    #122470

    Purpose

    Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 isoform C CRISPR resistant (SCAF8 Human)
    

    Use

    Tags

    FLAGHA

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. XE7 Xwnt-8

    Plasmid

    #16869

    Depositor

    Randall Moon

    Article

    Christian et al Development. 1991 Apr. 111(4):1045-55.

    Insert

    Xwnt-8 RNA (wnt8a.L Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

26. Cav1-mRed

    Plasmid

    #12681

    Depositor

    Richard Pagano

    Article

    Sharma et al Mol Biol Cell. 2004 Jul . 15(7):3114-22.

    Insert

    canine caveolin-1 (CAV1 Canis familiaris)
    

    Use

    Tags

    mRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pDS271

    Plasmid

    #34974

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A, V416I

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMH-HT_SsCHAD

    Plasmid

    #127783

    Purpose

    Expresses SsCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p667-UBC-DYRK2-FullLength-V5-miniTurbo_IDG-K

    Plasmid

    #198010

    Purpose

    Gateway destination clone of DYRK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    DYRK2-V5-miniTurbo (DYRK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-2TK-YAP-Ag

    Plasmid

    #19063

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

31. pFRT/TO/GFP-SCAF8 (CRISPR_resistant)

    Plasmid

    #122472

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF8 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 (SCAF8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5-hygro-eGFP-HEC1-9D

    Plasmid

    #114029

    Purpose

    Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9D (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 9D

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. N6AMT1_pCSdest

    Plasmid

    #53843

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    N6AMT1 (N6AMT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

34. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAP1836

    Plasmid

    #137087

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pChlr-7SE

    Plasmid

    #52168

    Purpose

    Encodes module 7 with amino acids 12S-16E for recognition of nt 2G

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFETCh_NCoA2

    Plasmid

    #86283

    Purpose

    Donor vector for 3' FLAG tag of human NCoA2

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NCoA2 homology arms (NCOA2 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CAV1-D67G-HA

    Plasmid

    #27706

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutant D67G

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGL3-PIM2

    Plasmid

    #51848

    Purpose

    promoter region (-550;0) of PIM2

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    PIM2 (PIM2 Human)
    

    Use

    Luciferase; Reporter vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA4/HisMaxB-YAP2

    Plasmid

    #18997

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only