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We narrowed to 18,759 results for: nls

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Showing: 3041 - 3080 of 18759 results

  1. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enOsCas12f1
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197026-plasmid-map-sequence-id-394112

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  5. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

    Plasmid
    #133798
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19
    Insert
    human codon optimized AcrIIA1(T114A/F115A)
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Mutation
    T114A/F115A
    Promoter
    CMV and T7
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    133798-plasmid-map-sequence-id-268051

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  8. pEGFP-C1-AR Q0

    Plasmid
    #86427
    Purpose
    Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region
    Depositor
    Michael Mancini
    Publication
    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    androgen receptor (AR) (AR Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of the poly-glutamine expansion
    Promoter
    CMV
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86427-plasmid-map-sequence-id-374317

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  9. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid
    #114093
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114093-plasmid-map-sequence-id-230492

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  11. 3xFN-Tel-TAL/pMXs-puro

    Plasmid
    #63590
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Promoter
    LTR
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63590-plasmid-map-sequence-id-108379

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  15. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

    Plasmid
    #172195
    Purpose
    AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Publication
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    transcription regulatory domain removed
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    172195-plasmid-map-sequence-id-343564

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  18. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enRhCas12f1
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197027-plasmid-map-sequence-id-388964

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  19. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47869
    Purpose
    All-in-one plasmid targeting tdTomato
    Depositor
    Erik Sontheimer, James Thomson
    Publication
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    NmCas9
    U6pr-tracrRNA
    tdTomato crRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47869-plasmid-map-sequence-id-68867

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  23. pL6puro-JeT-pmRevErbA-mScaI3-NLS-Pest

    Plasmid
    #240107
    Purpose
    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added JeT Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240107-plasmid-map-sequence-id-481763

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  29. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114082-plasmid-map-sequence-id-220504

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  30. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

    Plasmid
    #115486
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115486-plasmid-map-sequence-id-241911

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  31. pFC482

    Plasmid
    #246439
    Purpose
    The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal
    Depositor
    Ron Weiss
    Publication
    Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038
    Insert
    CasE
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SapI domesticated
    Promoter
    EF-1a
    Available since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246439-plasmid-map-sequence-id-492325

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  33. APEX2 VLP

    Plasmid
    #182211
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182211-plasmid-map-sequence-id-357716

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  36. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid
    #176458
    Purpose
    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176458-plasmid-map-sequence-id-346303

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  38. p4B5-EGFP

    Plasmid
    #140324
    Purpose
    Detection of Dengue virus infection in live cells
    Depositor
    Alan Rothman
    Publication
    Medin et al J Virol Methods. 2015 Jan;211:55-62. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    2 conservative n.t. differences near 5' end …
    Promoter
    CMV
    Available since
    May 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    140324-plasmid-map-sequence-id-296784

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  39. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202042
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Publication
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-1xNLS-[Activ]
    Expression
    Mammalian
    Available since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202042-plasmid-map-sequence-id-401647

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  40. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202043
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Publication
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-[Activ]
    Expression
    Mammalian
    Available since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202043-plasmid-map-sequence-id-401604

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Showing: 3041 - 3080 of 18759 results