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Showing: 3851 - 3900 of 22681 results

  26. pInducer10b-HA-KRAS WT CO SR

    Plasmid
    #164926
    Purpose
    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence
    Depositor
    Ji Luo
    Article
    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.
    Insert
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mutation
    9 Valine codons are optimized and siRNA targetin…
    Promoter
    Ubc promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    164926-plasmid-map-sequence-id-333718

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  29. pInducer10b-HA-KRAS G12V CO SR

    Plasmid
    #164928
    Purpose
    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence
    Depositor
    Ji Luo
    Article
    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.
    Insert
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mutation
    Glycine 12 changed to valine; 9 Valine codons are…
    Promoter
    Ubc promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    164928-plasmid-map-sequence-id-333719

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  39. pNLF1-C_PLCG1:1-1291

    Plasmid
    #211089
    Purpose
    Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    PLCG1:M1-L1291 (PLCG1 Human)
    Use
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Mutation
    isoform 2. I813T natural variant (VAR_011908)
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211089-plasmid-map-sequence-id-425967

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  42. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI-D10R-E48R
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60367-plasmid-map-sequence-id-456413

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  44. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170280-plasmid-map-sequence-id-334954

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  45. pRDA_122

    Plasmid
    #208095
    Purpose
    Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq
    Depositor
    John Doench
    Article
    Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.
    Insert
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208095-plasmid-map-sequence-id-405878

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  46. mitoTALECD for_-20G_1333CN

    Plasmid
    #186200
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186200-plasmid-map-sequence-id-368306

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  47. mitoTALECD for_ -13G_1397CN

    Plasmid
    #186199
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186199-plasmid-map-sequence-id-368290

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  48. mitoTALECD for_ -6G_1333CN

    Plasmid
    #186198
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186198-plasmid-map-sequence-id-368288

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Showing: 3851 - 3900 of 22681 results