We narrowed to 7,283 results for: Tet-on

1. p-dCas9-dTET1-H1672Y-D1674A-Hygro

   Plasmid

   #104413

   Purpose

   transient expression of dCas9-dTET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dTET1
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   H1672Y-D1674A

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAT-TET-OFF -MET - 3xHA

   Plasmid

   #182526

   Purpose

   Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   7 Copies of TetO Box
   

   Tags

   3HA

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBtet-Bla/PAG1-TurboID-GAI

   Plasmid

   #198217

   Purpose

   Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

   Depositor

   Eric Davis

   Insert

   PAG1 (PAG1 Human)
   

   Use

   Transposon

   Tags

   3’ part of TurboID, starting with G257 and amino …

   Expression

   Mammalian

   Mutation

   None

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-O-FUW-NR3C1-P2A-mCherry

   Plasmid

   #203854

   Purpose

   Tet-inducible lentiviral plasmid expressing NR3C1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   NR3C1 (NR3C1 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-TetCMV-puro-mCerulean-PA-Rac1

   Plasmid

   #22031

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

6. NAT-TET-OFF -LEU- 4xFLAG

   Plasmid

   #182527

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   7 Copies of TetO Box
   

   Tags

   4XFLAG

   Expression

   Yeast

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-Tet-puro-hARAF-shRNA-1

   Plasmid

   #185370

   Purpose

   For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF

   Depositor

   Ayaz Najafov

   Insert

   ARAF (ARAF Human)
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

   Plasmid

   #189955

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_MMERVK10c
   

   Use

   Lentiviral

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK-flag-GFP 1-10 tetra Cys

   Plasmid

   #78589

   Purpose

   Expression flag-GFP 1-10 tetra Cys in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-GFP 1-10 tetra Cys
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pFA6a-kanMX-tetO-Pcyc1-GST

    Plasmid

    #41026

    Depositor

    Michael Nick Boddy

    Article

    Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

    Insert

    tetO7-Pcyc1-GST
    

    Use

    N-terminal tagging/promoter replacement

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-FullLength-TET1-CXXC/LLAA

    Plasmid

    #124399

    Purpose

    Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    CXXC zinc finger deletion (aa 585-624) and L897A/…

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEST-p15A-PLtetO1-deGFP-T500

    Plasmid

    #45392

    Depositor

    Vincent Noireaux

    Article

    Shin et al J Biol Eng. 2010 Jun 24;4:8.

    Insert

    deGFP
    

    Expression

    Bacterial

    Promoter

    PL-tetO1

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-kanMX-tetO-Pcyc1-3xHA

    Plasmid

    #41024

    Depositor

    Michael Nick Boddy

    Article

    Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

    Insert

    tetO7-Pcyc1-3xHA
    

    Use

    N-terminal tagging/promoter replacement

    Available Since

    Dec. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1a-tetR-T2A-tSapphire

    Plasmid

    #176312

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    T-sapphire
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH227-Tier1-OTetO7-PCMVmin2-SEAP

    Plasmid

    #169563

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP production
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. plko-tet-on-puromycin-shTRX1-259

    Plasmid

    #110920

    Purpose

    To knockdown Thioredoxin-1 following doxycycline addition

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Lentiviral

    Promoter

    unknown

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TET-pLKO-neo CXCR4-sh3

    Plasmid

    #163743

    Purpose

    Lentivirus for inducible knockdown of CXCR4 (human)

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TET-pLKO-neo CXCR4-sh1

    Plasmid

    #163741

    Purpose

    Lentivirus for inducible knockdown of CXCR4 (human)

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TET-pLKO-neo CXCR4-sh2

    Plasmid

    #163742

    Purpose

    Lentivirus for inducible knockdown of CXCR4 (human)

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4-Tet-on-vsv-Incenp-EGFP

    Plasmid

    #108483

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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