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pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Expression

Mammalian

Promoter

CMV and T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Expression

Mammalian

Promoter

CMV and T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Glycine max, Mouse)

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pTet-O-RUNX3-T2A-PuroR

Plasmid

#162349

Purpose

Lentiviral expression of RUNX3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

RUNX3 (RUNX3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

Plasmid

#194278

Purpose

Expresses gRNA and dSaCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized dSaCas9 KRAB T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PAmCherry-N1-FRB-Tubbyc

Plasmid

#202678

Purpose

Photoactivatable and rapamycin recruitable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

Plasmid

#138983

Purpose

IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

Plasmid

#138990

Purpose

IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

Plasmid

#138988

Purpose

IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

Plasmid

#138987

Purpose

IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

Plasmid

#138986

Purpose

IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

Plasmid

#138985

Purpose

IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

Plasmid

#138984

Purpose

IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pL6puro-pSV40(1)-pmRevErbA-mScaI3-NLS-Pest

Plasmid

#240116

Purpose

A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Mutation

added SV40-Enhancer

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest

Plasmid

#240111

Purpose

A lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Mutation

added JeT Promoter

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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FL MALT1 WT in pET29b-Strep

Plasmid

#48968

Purpose

Expresses Full length wild type MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Tags

His and Strep

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pDONR221 NLRP1

Plasmid

#166823

Purpose

Gateway pDONR vector containing NLRP1 (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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