161,547 results

1. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Use

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-ERP2-(N-HA)KRAS_G12D

   Plasmid

   #201254

   Purpose

   Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

   Depositor

   Alexander Kleger

   Article

   Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

   Insert

   KRAS (KRAS Human)
   

   Use

   Piggybac transposon

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   changed Gly (G) 12 to Asp (D)

   Promoter

   TRE

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_ABEmax_P2A_GFP

   Plasmid

   #112101

   Purpose

   A:T-to-G:C base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   ABEmax_P2A_GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29-bdSUMO-Anti-ALFA nanobody

   Plasmid

   #212311

   Purpose

   Expression of anti-ALFA nanobody

   Depositor

   Arthur Laganowsky

   Article

   Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-hMLH1dn

   Plasmid

   #174824

   Purpose

   Mammalian expression of human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (codon optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_AncBE4max_P2A_GFP

   Plasmid

   #112100

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max_P2A_GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

   Plasmid

   #121966

   Purpose

   Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   superfold GFP-GB1-NLS-iLID

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-TetOne-Puro-LUC

   Plasmid

   #218630

   Purpose

   This plasmid contains luciferase under doxycycline inducible control.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   LUC (LOC116160065 )
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

   Plasmid

   #197501

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpyCatcher003-sfGFP

    Plasmid

    #133449

    Purpose

    Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003-sfGFP
    

    Use

    Tags

    His6 and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXR002: EF1a-dCasRx-2A-EGFP

    Plasmid

    #109050

    Purpose

    Catalytically inactive dCasRx with 2A-EGFP

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Zeo-mEGFP

    Plasmid

    #214145

    Purpose

    Expresses the monomeric EGFP in eukaryotic cells

    Depositor

    Bertrand Collet

    Article

    Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A207K L221K F223R

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti Lifeact-EGFP BlastR

    Plasmid

    #84383

    Purpose

    Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnCasPA-BEC

    Plasmid

    #113349

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_ENVLPE+

    Plasmid

    #232427

    Purpose

    Expression plasmid to produce ENVLPE+ particles

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    ENVLPE+
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xCMV

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Ii-OVA.I+II

    Plasmid

    #64596

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 242-353 (class I and class II ep…

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti6-H2B-mCherry

    Plasmid

    #89766

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    Histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-GFP11-Clathrin light chain

    Plasmid

    #70217

    Purpose

    Expresses GFP11-clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11-Clathrin light chain (CLTA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP(C3)-Nup153

    Plasmid

    #64268

    Purpose

    GFP-tagged mammalian expression of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    NuP153 (NUP153 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1 Lifeact-EGFP

    Plasmid

    #58470

    Purpose

    Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Lifeact
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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