We narrowed to 5,602 results for: pcas

1. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG dCas9-KRAB-2A-EGFP

   Plasmid

   #92396

   Purpose

   CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9_KRAB_2A_EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. spCas9-BlastR (pCBhCas9-BlastR)

   Plasmid

   #71489

   Purpose

   Expresses SpCas9 and Blasticidin resistance

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Insert

   spCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CBh

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-MLC2(CA)-IRES-GFP

   Plasmid

   #133928

   Purpose

   Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EGFP and MLC2(CA) (MYL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MLC2 T18D, S19D

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-NRCH-ABE8.17-m+V106W

   Plasmid

   #198551

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS SARS-CoV-2 BA.1 Spike

   Plasmid

   #185452

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

   Depositor

   Marceline Côté

   Article

   Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

   Insert

   SARS-CoV-2 Spike BA.1 (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: A67V, Δ69-70, T…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCAGGS-N-TEV-Twin-Strep

   Plasmid

   #202526

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site and Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas-pML-1B_6xHis-Cas12m

   Plasmid

   #192282

   Purpose

   MmCas12m fused to His-tag for protein purification

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12m
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9-EcRT-GAL-HIS3

    Plasmid

    #232093

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9-EcRT-GAL-Nat

    Plasmid

    #232090

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9-EcRT-GAL-Kan

    Plasmid

    #232092

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-tomato SOX2-sgRNA

    Plasmid

    #196190

    Purpose

    Editing human SOX2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-C-TEV-Twin-Strep

    Plasmid

    #202527

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Oct3/4-IRES-Neo

    Plasmid

    #13461

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P31S

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS SARS-CoV-2 BA.2 Spike

    Plasmid

    #185604

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

    Insert

    SARS-CoV-2 BA.2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    July 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-TET3G-T2A-Puro

    Plasmid

    #158067

    Purpose

    Encodes the Tet3G transactivator and puromycin resistance.

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    pCAG-TET3G-T2A-Puro
    

    Use

    AAV

    Tags

    puromycin reistance gene

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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