8,723 results

1. pYLsgRNA-OsU6b

   Plasmid

   #66196

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   OsU6b

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. HRE-luciferase

   Plasmid

   #26731

   Purpose

   Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

   Depositor

   Navdeep Chandel

   Article

   Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

   Insert

   Hypoxia Responsive Elements (3)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC013 - Twinstrep-SUMO-huLwCas13a

   Plasmid

   #90097

   Purpose

   Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   6xHis-Twin Strep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. EF.STAT3DN.Ubc.GFP

   Plasmid

   #24984

   Purpose

   Depositor

   Linzhao Cheng

   Article

   Hillion et al Cancer Research 68, 10121, December 2008

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y705F--dominant negative

   Promoter

   Available since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. G5-CD40

   Plasmid

   #121166

   Purpose

   pMP71 vector expressing GFP - P2A - G5 - myc - CD40

   Depositor

   Gabriel Victora

   Article

   Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

   Insert

   G5-CD40
   

   Use

   Retroviral

   Tags

   GFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRhoA2G

   Plasmid

   #40179

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-RhoA FLARE.sc Biosensor WT

   Plasmid

   #12150

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-jGCaMP8s

   Plasmid

   #162371

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8s
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRL623

   Plasmid

   #58494

   Purpose

   helper plasmid for bacterial conjugal DNA transfer

   Depositor

   Peter Wolk

   Article

   Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

   Insert

   encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry2-C1

    Plasmid

    #54563

    Purpose

    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi-RP

    Plasmid

    #60513

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRFP-Lact-C2

    Plasmid

    #74061

    Purpose

    Fluorescent biosensor for phosphatidylserine.

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 (MFGE8 Bovine)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

    Plasmid

    #134868

    Purpose

    Expresses EGFP-tagged lysosomal marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLV-ER GFP

    Plasmid

    #80069

    Purpose

    To express and target GFP into the endoplasmic reticulum using lentivirus

    Depositor

    Pantelis Tsoulfas

    Insert

    ER GFP (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    GFP derived from Aequorea coerulescens on the NH2…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GAL4UAS

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-NbALFA

    Plasmid

    #159987

    Purpose

    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mCherry-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mCherry and NbALFA

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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