We narrowed to 24,628 results for: Nes

1. pAureus-TnCAM

   Plasmid

   #199807

   Purpose

   vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

   Depositor

   Steve Salipante

   Article

   Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

   Insert

   transposon cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBabe NEO mRFP1

   Plasmid

   #98397

   Purpose

   Retroviral vector for constitutive mRFP1 expression

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   mRFP1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-luxKate

   Plasmid

   #229951

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxKate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMX/cyclinB1-GFP (27)

   Plasmid

   #26061

   Depositor

   Jonathon Pines

   Article

   Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330A_dCas9-1x7

   Plasmid

   #63601

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GST HP1 gamma

   Plasmid

   #24076

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 gamma (CBX3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Expression

   Mutation

   Promoter

   TgTUB1 and Toxoplasma U6

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSWEET-bgaB

   Plasmid

   #210272

   Purpose

   Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

   Depositor

   Eric Brown

   Article

   Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

   Insert

   bgaB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PxylA

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-mEOS3.2-KanMX6

   Plasmid

   #87030

   Purpose

   Tag S. pombe genes in C terminal with mEOS3.2

   Depositor

   Thomas Pollard, Julien Berro

   Article

   Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   mEOS3.2
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ReNL/pcDNA3

    Plasmid

    #85203

    Purpose

    Red color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Red enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-luxKOk

    Plasmid

    #229949

    Purpose

    Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxKOk
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330S-7

    Plasmid

    #58783

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDR FLIP43 GPS1

    Plasmid

    #101084

    Purpose

    GA biosensor expression plasmid

    Depositor

    Wolf Frommer, Alexander Jones

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    GPS1
    

    Use

    Tags

    6HIS and cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5'Entry-lck-7.4kb

    Plasmid

    #58891

    Purpose

    provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 1874th nucle…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GST HP1 beta

    Plasmid

    #24075

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 beta (CBX1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pX330S-4

    Plasmid

    #58780

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Orange_CaMBI_110

    Plasmid

    #124094

    Purpose

    orange calcium-modulated bioluminescent indicator, 110nM

    Depositor

    Michael Lin

    Article

    Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

    Insert

    Orange CaMBI 110
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS-TarHom-Tol2-kanaR-cryaa-CFP

    Plasmid

    #74151

    Purpose

    BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    Tol2-kanaR-cryaa-CFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMC-6-StayGold-9

    Plasmid

    #197734

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    StayGold GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YeNL/pcDNA3

    Plasmid

    #85201

    Purpose

    Yellow color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Yellow enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pSECRETS-A

    Plasmid

    #196986

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pltetO1

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSGV Hu Acceptor

    Plasmid

    #64269

    Purpose

    Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJSK663

    Plasmid

    #205129

    Purpose

    Expression of GFP-1xPX (from SnxA) in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    1xPX domain of SnxA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA HA HP1 beta

    Plasmid

    #24079

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 beta (CBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

25. CeNL/pcDNA3

    Plasmid

    #85199

    Purpose

    Cyan color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Cyan enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBAD-luxOFP

    Plasmid

    #229945

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxOFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMC-1-StUbi-6

    Plasmid

    #197710

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    StUbi promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RP4-6

    Plasmid

    #80385

    Purpose

    Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Spectinomycin/Streptomycin
    

    Use

    Helper bhr plasmid for mobilizing other plasmids …

    Tags

    none

    Expression

    Mutation

    Promoter

    None

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pBAD-luxKate

    Plasmid

    #229946

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxKate
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330A-1x3

    Plasmid

    #58767

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMaCTag-Z31

    Plasmid

    #120074

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    V5
    

    Use

    Pcr template

    Tags

    V5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RP4-11

    Plasmid

    #79812

    Purpose

    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Kanamycin cassette and SacB gene (sacB )
    

    Use

    Helper plasmid for mobilization

    Tags

    Expression

    Mutation

    Promoter

    Km

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

33. OeNL/pcDNA3

    Plasmid

    #85202

    Purpose

    Orange color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Orange enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

34. pSIM24 KanR

    Plasmid

    #67583

    Purpose

    Plant Gene Expression Vector

    Depositor

    Indu Maiti

    Article

    Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    M24

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX330S-5

    Plasmid

    #58781

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMC-6-IPT-9

    Plasmid

    #197746

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Isopentyl transferase (IPT)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ReNL_pRSETb

    Plasmid

    #89536

    Purpose

    Red color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p16 nlsGPS1

    Plasmid

    #101081

    Purpose

    GA biosensor expression plasmid

    Depositor

    Wolf Frommer, Alexander Jones

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    nlsGPS1
    

    Use

    Tags

    cMyc

    Expression

    Plant

    Mutation

    Promoter

    p16 (AT3G60245)

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-Orange_CaMBI_300

    Plasmid

    #124095

    Purpose

    orange calcium-modulated bioluminescent indicator, 300nM

    Depositor

    Michael Lin

    Article

    Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

    Insert

    Orange CaMBI 300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBAD-luxKOk

    Plasmid

    #229944

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxKOk
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge