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Showing: 3901 - 3950 of 32215 results

  9. pET28a-RBP-LOV2

    Plasmid
    #185792
    Purpose
    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker
    Depositor
    Lizhong He
    Article
    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864
    Insert
    RBP-LOV2
    Use
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185792-plasmid-map-sequence-id-367546

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  16. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Tags
    Expression
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

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  31. CMV-mito-CAR-GECO1

    Plasmid
    #46022
    Purpose
    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    CAR-GECO1
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid
    #105200
    Purpose
    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    105200-plasmid-map-sequence-id-201271

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  39. pmCherry-CIBN-CreC

    Plasmid
    #26889
    Purpose
    Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.
    Depositor
    Chandra Tucker
    Article
    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:
    Insert
    CIBN-CreC
    Use
    Tags
    Expression
    Mammalian
    Mutation
    aa 1-170 of CIB1, aa 106-343 of Cre
    Promoter
    CMV
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    26889-plasmid-map-sequence-id-12741

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  42. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Use
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  45. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Use
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37276-plasmid-map-sequence-id-46873

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  47. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

    Plasmid
    #111924
    Purpose
    Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    OeNL(Ca2+)_18μ
    Use
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111924-plasmid-map-sequence-id-214007

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Showing: 3901 - 3950 of 32215 results