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Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F0
Plasmid#184162PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Ptdh3-CAD-Ttdh1
Plasmid#127727PurposeYeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtdh3Available sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F1
Plasmid#184163PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-AsNPGA-9
Plasmid#197751PurposePhytobrick (MoClo) Level 0 PartDepositorInsertFBP: AsNPGA (luciferin synthesis)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ATA-P(11)4
Plasmid#185787PurposeEncodes enzyme Ļ-aminotransferase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertATA-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglA
Plasmid#170720PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)DepositorInsertRhamnogalacturonan lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPEE42
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE45
Plasmid#139424PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.DepositorInsertmTurquoise1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE44
Plasmid#139423PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.DepositorInsertmTFP1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE43
Plasmid#139422PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.DepositorInsertmMaroon1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tadh1
Plasmid#127723PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Ppre3-ITE-Teno1
Plasmid#127724PurposeYeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpre3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tdpp1
Plasmid#127725PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Phta1-ITE-Tvma2
Plasmid#127726PurposeYeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPhta1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFBP_2xNbLYS1::LUZ
Plasmid#189918PurposeBinary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.DepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromoterVariousAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 ā¦PromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
UseTags6xHisExpressionYeastMutationG951A,C957APromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-CPR
Plasmid#126742Purposeyeast plasmid overexpressing CPRDepositorInsertcpr
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRI012-pGEM-PacI-PU3-BsmbI-NotI
Plasmid#140204PurposeVector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.DepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Step 1 of cloning cā¦TagsExpressionMutationPromoterAspergillus fumigatus U3 (RNAPIII).Available sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2357_Tier3(PB)-TagBFP_BlastR
Plasmid#169656PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-sAR
Plasmid#126743Purposeyeast plasmid overexpressing CPR and sLovADepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrps3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeft2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDRM98 mKate2 Blast
Plasmid#174720PurposeExpresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.DepositorInsertsmKate2
BSD
UseLentiviralTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND and MND (off mKate-P2A)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-LovC
Plasmid#126729Purposeyeast plasmid overexpressing LovCDepositorInsertlovC
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-LovB
Plasmid#126728Purposeyeast plasmid overexpressing LovBDepositorInsertlovB
UseTags6xHisExpressionYeastMutationT7227A,T8994APromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFC902
Plasmid#106904PurposeThe vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1DepositorInsertscas9
pyrG
U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR
Plasmid#160046PurposeFor expressing the full paradoxical circuitDepositorInsertsosTIR1
iaaH
iCasp9
BlastR
UseSynthetic BiologyTagsmAID, mCherry, and mGFPmut3ExpressionMammalianMutationPromoterno promoter, T2A is used and pEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FB009
Plasmid#119707PurposeTranscriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPtrpC::nptII::Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-LM_lacO-cP-AOX1-sAR
Plasmid#126745Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-ICL1-LM_lacO-cP-AOX1-sAR
Plasmid#126744Purposeyeast plasmid overexpressing sLovA,CPR and LacI-Mit1ADDepositorInsertslovA,cpr
UseTags6xHisExpressionYeastMutationPromoterICL1Available sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKatā¦Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCHKU27.1-2.2
Plasmid#111048PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertPKS
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHKU27.2-2.2
Plasmid#110662PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only