We narrowed to 201 results for: KLF4

1. PB-TAC-OKMS-KRAB-dCas9

   Plasmid

   #120356

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-OSKM

   Plasmid

   #80481

   Purpose

   piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetOminiCMV-OSKM

   Plasmid

   #136541

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TET-MKOS

   Plasmid

   #20959

   Purpose

   piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Available Since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TAC-MKOS

   Plasmid

   #80484

   Purpose

   piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-OK+9MS-Cas9

   Plasmid

   #120354

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-hK + E2-Crimson

   Plasmid

   #74946

   Purpose

   Dosage control and tracing of reprogramming episomes

   Depositor

   Ann Zovein

   Article

   Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

   Insert

   Klf4 (KLF4 Human)
   

   Expression

   Mammalian

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

8. PB-TAC-OK+9MS-KRAB-dCas9

   Plasmid

   #120357

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

   Plasmid

   #176027

   Purpose

   construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   rtTA3
   Luc2
   mtagBFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIN4-CMV-K2M

    Plasmid

    #21164

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Klf4, cMyc (MYC, KLF4 Human)
    

    Use

    Lentiviral; Reprogramming

    Expression

    Mammalian

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLM-mCerulean-cMyc

    Plasmid

    #23244

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nanog-luciferase targeting construct

    Plasmid

    #26591

    Depositor

    Rudolf Jaenisch

    Article

    Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

    Insert

    Nanog promoter-luciferase-lox-pgk-neomycin-loc
    

    Expression

    Bacterial

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4-Cluster Promoter Delta 1

    Plasmid

    #100126

    Purpose

    It contains ~1.4Kb of the miR-183/96-182 cluster promoter

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    miR-183-96-82 Cluster promoter (MIR182 Human)
    

    Use

    Luciferase

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4 Cluster Promoter Delta 1.2

    Plasmid

    #100130

    Purpose

    It contains 0.5 Kb of the miR-183-96-182 cluster promoter. This fragment partially loses promoter activity in melanoma cells.

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    miR-183-96-82 Cluster promoter 0.5 Kb Fragment (MIR182 Human)
    

    Use

    Luciferase

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4-Cluster Promoter Delta 2

    Plasmid

    #100127

    Purpose

    It contains ~4.8 Kb of the miR-183-96-182 cluster promoter

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    miR-183-96-82 Cluster promoter 4.8Kb Fragment (MIR182 Human)
    

    Use

    Luciferase

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4-Cluster Promoter Delta 3

    Plasmid

    #100128

    Purpose

    It contains ~2.7 Kb of the miR-183-96-182 cluster promoter

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    miR-183-96-82 Cluster promoter 2.7Kb Fragment (MIR182 Human)
    

    Use

    Luciferase

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4-Cluster Promoter Delta 1.1

    Plasmid

    #100129

    Purpose

    It contains ~0.8 Kb of the miR-183-96-182 cluster promoter. Is the minimal region that contains the maximal promoter activity

    Depositor

    Eva Hernando

    Article

    Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

    Insert

    miR-183-96-82 Cluster promoter 0.8 Kb Fragment (MIR182 Human)
    

    Use

    Luciferase

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. T7-VEE-OKS-iG

    Plasmid

    #58974

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEP4 E02S CK2M EN2L

    Plasmid

    #20924

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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