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pET-15b-EK_C122S_His5

Plasmid

#49048

Purpose

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

Depositor

Hans Brandstetter

Publication

Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

Insert

Enteropeptidase, peptidase domain (TMPRSS15 Bovine)

Tags

5xHis

Expression

Bacterial

Mutation

P92R, E185D, C122S

Promoter

T7

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223-JAK2

Plasmid

#23915

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

JAK2 (JAK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Publication

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Publication

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

83 Articles

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Publication

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pLM-CMV-R-Cre

Plasmid

#27546

Purpose

Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

Depositor

Michel Sadelain

Publication

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

mCherry_P2A_Cre recombinase

Use

Cre/Lox and Lentiviral

Promoter

CMV

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Publication

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMXs-Stat3-C

Plasmid

#13373

Purpose

Retroviral expression of dominant active mouse Stat3

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Changed Alanine 661 to cysteine and asparagine 66…

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pKD4

Plasmid

#45605

Depositor

Barry L. Wanner

Publication

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-kan-FRT

Expression

Bacterial

Promoter

R6K

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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AAV-GFP/Cre

Plasmid

#49056

Purpose

Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

Depositor

Fred Gage

Publication

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, Myc, and NLS

Expression

Mammalian

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pRSET-RCaMP1h

Plasmid

#42874

Purpose

a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

Depositor

Loren Looger

Publication

Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

Insert

Red Fluorescent Calcium binding Protein

Tags

EK (Enterokinase) Recognition Site, His Tag (6x),…

Expression

Bacterial

Promoter

T7

Available since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3-EGFP-Rac1-T17N

Plasmid

#12982

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rac1 DN (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pBabe puro Myr HA PIK3CA

Plasmid

#12523

Depositor

Jean Zhao

Publication

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA Myr (PIK3CA Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele; I14…

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCAG-Cre-IRES2-GFP

Plasmid

#26646

Purpose

Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

Depositor

Anjen Chenn

Publication

Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

Insert

Cre

Use

Cre/Lox

Tags

GFP and IRES2

Expression

Mammalian

Promoter

CAG

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pT7-PEmax for IVT

Plasmid

#178113

Purpose

Template for in vitro transcription of PEmax

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PEmax-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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PKC alpha WT

Plasmid

#21232

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Publication

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pCHAC-mt-mKeima

Plasmid

#72342

Purpose

retrovirus construct for stable expressing mt-mKeima

Depositor

Richard Youle

Publication

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

mt-mKeima

Use

Retroviral

Available since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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