19,618 results

1. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

   Plasmid

   #71237

   Purpose

   Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A GFP
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK-ATF3

   Plasmid

   #26115

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF3 (ATF3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAS-NanoLuc

   Plasmid

   #87696

   Purpose

   Gal4VP16 driven expression of NanoLuc

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NanoLuc
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW mCherry-GFP-LC3

   Plasmid

   #110060

   Purpose

   To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

   Depositor

   Anne Brunet

   Article

   Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

   Insert

   mCherry-GFP-LC3
   

   Use

   Lentiviral

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. murine TCR OTII-2A.pMIG II

   Plasmid

   #52112

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   P2A (from porcine teschovirus-1)

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330.puro

   Plasmid

   #110403

   Purpose

   CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-p65

   Plasmid

   #111190

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

   Insert

   RELA (RELA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV.CamKII.GCaMP6s.WPRE.SV40

    Plasmid

    #107790

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG BacMam

    Plasmid

    #160451

    Purpose

    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    lac promoter / tac promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-Kras G12D

    Plasmid

    #58902

    Purpose

    Expresses human Kras with a G12D Mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12D mutation

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

    Plasmid

    #31482

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce Target) EF Puro
    

    Use

    Lentiviral

    Mutation

    mCherry has M9S and M16L mutations. This is what …

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Luc

    Plasmid

    #45968

    Depositor

    Ralf Kuehn

    Insert

    Luciferase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCXLE-hOCT3/4

    Plasmid

    #27076

    Purpose

    Non-integrating (episomal) expression of human OCT3/4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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