Addgene

We narrowed to 6,446 results for: poly

Showing: 2401 - 2430 of 6446 results

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pQE-9-RTXWTM-X2D-C

Plasmid

#225969

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of aspartic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2D-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2E-C

Plasmid

#225970

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of glutamic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2E-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2A-C

Plasmid

#225971

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGADTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTMCR-TLY-X2A-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2H-C

Plasmid

#225972

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGHDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2H-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2S-C

Plasmid

#225973

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGSDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2S-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2N-C

Plasmid

#225974

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGNDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2N-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2D-C

Plasmid

#225975

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGDDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2D-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXMCR-TLY-X2E-C

Plasmid

#225976

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGEDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2E-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2A-C

Plasmid

#225965

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of alanine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2A-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-9-RTXWTM-X2H-C

Plasmid

#225966

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of histidine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2H-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAM298

Plasmid

#224257

Purpose

MCM WT ubiquitin

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MCM Ubiquitin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAM305

Plasmid

#226137

Purpose

Expression of His6-mEos3.2-UbK48R

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-mEos3.2-UbK48R

Use

Tags

Expression

Bacterial

Mutation

K48R

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAM302

Plasmid

#226135

Purpose

Expression of MC-Ub-UbK48R-SH3-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-UbK48R-SH3-3C-His6

Use

Tags

Expression

Bacterial

Mutation

K48R

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAM303

Plasmid

#226136

Purpose

Expression of His6-GG-UbK48R

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GG-UbK48R

Use

Tags

Expression

Bacterial

Mutation

K48R

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM294

Plasmid

#226130

Purpose

Expression of Ufd1-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1-3C-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Fusion of West Nile NS2B-NS3 proteins

Plasmid

#204796

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204790

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro

Use

Tags

Expression

Bacterial

Mutation

MSAVMQ/SGLVKM

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCfB12717

Plasmid

#212691

Purpose

integration vector for three gene cassette with integration site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12718

Plasmid

#212692

Purpose

integration vector for three gene cassette with integration site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12715

Plasmid

#212689

Purpose

integration vector for three gene cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB13017

Plasmid

#212693

Purpose

integration vector for three gene cassette with integration site of the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12716

Plasmid

#212690

Purpose

integration vector for three gene cassette with integration site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12714

Plasmid

#212688

Purpose

integration vector for three gene cassette with integration site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
WNV NS2B-NS3 protease: Co-expression of NS2B and NS3

Plasmid

#204806

Purpose

Bacterial co-expression of West Nile NS2B and NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-D68 2A protease; 6xHis-GST-TEV, low expression levels

Plasmid

#204804

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

Plasmid

#204811

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
RPO41

Plasmid

#205072

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 100 amino acids

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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SNAPf-mTurquoise2-KDEL

Plasmid

#197498

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LifeAct-SNAPf-mTurquoise2

Plasmid

#197497

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in actin filaments.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge