4,124 results

1. ER-mNeonGreen

   Plasmid

   #137804

   Purpose

   Visualization of the Endoplasmic Reticulum

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE TRE dCas9-VP64

   Plasmid

   #50916

   Purpose

   Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A nuclease-deficient

   Promoter

   TRE

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-7 asyn WT

   Plasmid

   #36046

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223-PDGFRB

   Plasmid

   #23893

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PDGFRB (PDGFRB Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223-EGFR

   Plasmid

   #23935

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Xiap-Myc

   Plasmid

   #11833

   Depositor

   Guy Salvesen

   Article

   Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

   Insert

   XIAP (XIAP Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-syn-jGCaMP7f-WPRE

   Plasmid

   #104488

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

   Insert

   jGCaMP7f
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV4-p100

   Plasmid

   #23287

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   NFkB p100 subunit (NFKB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mcherry-PH

   Plasmid

   #36075

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   PH domain of PLCdelta1 (PLCD1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-DsRed_IRES_EGFP

    Plasmid

    #92194

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdCas9-DNMT3A-EGFP

    Plasmid

    #71666

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLEM415-ldhL-mRFP1

    Plasmid

    #99842

    Purpose

    Expression of RFP in Lactobacillus

    Depositor

    Sujin Bao

    Article

    Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    ldhl

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUCas9Cherry

    Plasmid

    #70182

    Purpose

    Expresses Cas9 with fluorescent Cherry reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    humanised S.pyogenes cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBPp65ADZpUw

    Plasmid

    #26234

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    p65AD-Zip (RELA Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-TAZ

    Plasmid

    #32839

    Depositor

    Kunliang Guan

    Article

    Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. human p53-(1-393)

    Plasmid

    #24859

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Full length.

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-CAG-ChR2-GFP

    Plasmid

    #26929

    Purpose

    AAV-mediated expression of ChR2-GFP for neuronal excitation

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Insert

    ChR2
    

    Use

    AAV; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flag-HA-COPS5

    Plasmid

    #22541

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    COPS5 (COPS5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-TRE-EGFP

    Plasmid

    #89875

    Purpose

    EGFP reporter vector of iTango system for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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