We narrowed to 6,346 results for: rel

1. pCS2+-FLAG3-hsAgo2D-A

   Plasmid

   #221720

   Purpose

   Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2D-A (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   D669A in active site

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2+-FLAG3-hsAgo2

   Plasmid

   #221719

   Purpose

   Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2 (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+-FLAG3-drAgo2Y-F

   Plasmid

   #221715

   Purpose

   Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2Y-F (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Y680F in active site

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+-FLAG3-drAgo2D-E

   Plasmid

   #221714

   Purpose

   Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2D-E (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   D651E in active site

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFB-D3-ASK1

   Plasmid

   #226619

   Purpose

   Co-expression of D3 and ASK1 complex in insect cells

   Depositor

   Ning Zheng

   Article

   Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

   Inserts

   D3
   ASK1 (SKP1 Mustard Weed)
   

   Use

   Tags

   His tag followed with three copies of Msyb

   Expression

   Insect

   Mutation

   The flexible loop in D3 (Q478-D515) can be remove…

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBIOS1458

   Plasmid

   #171715

   Purpose

   expresses maize Glutamine Synthetase ZmGln1-3 cDNA under the control of the Cassava Vein Mosaic Virus promoter (CsVMV) (cassette 1) and the maize Rubisco small subunit promoter (ZmRbcS) (cassette 2)

   Depositor

   Christophe Sallaud

   Article

   Amiour et al Commun Biol. 2021 Sep 17;4(1):1095. doi:

   Insert

   ZmGln1-3
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

   Plasmid

   #215699

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

   Plasmid

   #206434

   Purpose

   Vasa 463-661 expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Vasa 463-661 (vas Fly)
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   F504E

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1-PH-mCherry-Oskar 139-240 MUT (A162E/L228E) (HK202)

   Plasmid

   #206464

   Purpose

   Oskar 139-240 mut expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Oskar 139-240 mut (osk Fly)
   

   Use

   Tags

   PH mcherry

   Expression

   Insect

   Mutation

   A162E L228E)

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFP-mVprBP pc2953

    Plasmid

    #201698

    Purpose

    Expresses of GFP-tagged VprBP in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    VprBP (Dcaf1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmcherry-mVprBP pc2954

    Plasmid

    #201699

    Purpose

    Expresses of mcherry-tagged mVprBP in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    VprBP (Dcaf1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3-TEX264wt-cFlag

    Plasmid

    #220204

    Purpose

    Mammalian expression of wild type TEX264, C-terminal Flag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3-TEX264-E194A-cFlag

    Plasmid

    #220207

    Purpose

    Mammalian expression of TEX264 with E194A mutation, C-terminal Flag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    194E>A

    Promoter

    CMV

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA5 TEX264wt-cGFP

    Plasmid

    #220211

    Purpose

    Mammalian expression of wild type TEX264 fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME map2k2a_s219D

    Plasmid

    #201058

    Purpose

    Gateway middle entry vector expressing constitutively active zebrafish map2k2a

    Depositor

    Sarah Childs

    Article

    Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

    Insert

    MAP2k2a (map2k2a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Mutation S219D, constitutively active

    Promoter

    none

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-cFOS-FRB-VP16-P2A-EGFP

    Plasmid

    #210510

    Purpose

    expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    FRB-VP16-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid

    #210514

    Purpose

    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    

    Use

    Tags

    HA signal FALG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Qt-FLAG-TGA-rt9us-antiGFP

    Plasmid

    #206954

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFSynW SYT9 D197, 199N IRES GFP

    Plasmid

    #195701

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full-length mouse SYT9 with D197, 199N mutations

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFSynW SYT9 D330, 332N IRES GFP

    Plasmid

    #195702

    Purpose

    Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full-length mouse SYT9 with D330, 332N mutations

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Qt-FLAG-TAA-rt20s-antiGFP

    Plasmid

    #206955

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 1M-Qt-FLAG-TGA-rt20s-antiGFP

    Plasmid

    #206953

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CAGGS-SAFB-NLS-3XFLAG-V5

    Plasmid

    #196089

    Purpose

    Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330_SAFBgRNA8

    Plasmid

    #196103

    Purpose

    Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196106, 196107, 196108

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX330_SAFB2gRNA12

    Plasmid

    #196106

    Purpose

    Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196107, 196108

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB2 (Safb2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330_SAFB2gRNA13

    Plasmid

    #196107

    Purpose

    Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196108

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB2 (Safb2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV_hSyn1_AdoLightG

    Plasmid

    #187465

    Purpose

    Expresses green adenosine indicator AdoLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AdoLightG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2/shCol1a1/Scarlet_Seq1.2

    Plasmid

    #201404

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT2/shCol1a1/Scarlet-Seq1.1

    Plasmid

    #201401

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT2/Col1a1/GFP4_Seq 1.1

    Plasmid

    #201400

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT2/sh-Col1a1/GFP4_Seq1.2

    Plasmid

    #201403

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAc5.1B-lambdaN-DmPABPC1-RRM1-RRM2-V5His6_H

    Plasmid

    #146490

    Purpose

    Insect Expression of DmPABPC1-RRM1-RRM2

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmPABPC1-RRM1-RRM2 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAc5.1B-lambdaN-DmPABPC1-RRM2-RRM3-V5His6_H

    Plasmid

    #146491

    Purpose

    Insect Expression of DmPABPC1-RRM2-RRM3

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmPABPC1-RRM2-RRM3 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-lambdaN-DmPABPC1-RRM3-RRM4-V5His6_H

    Plasmid

    #146492

    Purpose

    Insect Expression of DmPABPC1-RRM3-RRM4

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmPABPC1-RRM3-RRM4 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc5.1B-delLN-DmPABPC1-V5His6_H

    Plasmid

    #146477

    Purpose

    Insect Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmPABPC1 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one non silent mutation G26S, and seven silent mu…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-DmPABPC1-RRM1-RRM2-V5His6_H

    Plasmid

    #146479

    Purpose

    Insect Expression of DmPABPC1-RRM1-RRM2

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmPABPC1-RRM1-RRM2 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHEN-CoV2-nanobody-C7

    Plasmid

    #194591

    Purpose

    Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    C7 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDH-CMV-RPS15A-3xHA plasmid

    Plasmid

    #192245

    Purpose

    overexpression vector of RPS15A-3XHA

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    RPS15A CDS without stop coden (RPS15A Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. eSpCas9-EF-5Usp5

    Plasmid

    #174873

    Purpose

    CRISPR vector for generating Usp5 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Usp5 (Usp5 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. eSpCas9-EF-5Wnk1

    Plasmid

    #174874

    Purpose

    CRISPR vector for generating Wnk1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Wnk1 (Wnk1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. mKate2-DrCavin1b in pT3TS-Dest

    Plasmid

    #194295

    Purpose

    In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1b (cavin1b Zebrafish)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192230

    Purpose

    lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192229

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. RPS15A sgRNA-2 lentiCRISPR v2 plasmid

    Plasmid

    #192228

    Purpose

    lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. RPS15A sgRNA-1 lentiCRISPR v2 plasmid

    Plasmid

    #192227

    Purpose

    lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. mTln1-F2F3-P229L-pET151

    Plasmid

    #177879

    Purpose

    Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Leucine (P229L)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. mTln1-F2F3-P229S-pET151

    Plasmid

    #177878

    Purpose

    Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCS2-mCherry-Cenexin-S796A

    Plasmid

    #186434

    Purpose

    Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

    Insert

    Cenexin (ODF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Serine 796 to Alanine

    Promoter

    CMV IE94

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. BMP10 N67Q N131Q D4G (S2-2)

    Plasmid

    #184622

    Purpose

    for transfection into S2 cells and expression of BMP10 protein

    Depositor

    Timothy Springer

    Article

    Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

    Insert

    BMP10 (BMP10 Human)
    

    Use

    Tags

    8x His - streptavidin binding peptide

    Expression

    Insect

    Mutation

    N67Q, N131Q, ARIRR(312-316) replaced with G

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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