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pcDNA-Ikkb-FLAG WT

Plasmid

#23298

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

Ikkbeta (IKBKB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR3c-V5/HIS

Plasmid

#201108

Purpose

expression of human FGFR3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Npl4-Strep-HA

Plasmid

#113495

Purpose

Expression of human Npl4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Npl4 (NPLOC4 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-SUMO2-Q90P

Plasmid

#36448

Purpose

Expresses HA-Sumo2Q90P mutant in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO2 (SUMO2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Mutated Q90P

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
PDI-QUAD-V5-pcDNA3.1

Plasmid

#153550

Purpose

PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.

Depositor

Julie Atkin

Article

Parakh et al iScience. 2020 May 22;23(5):101097. doi:

Insert

Protein disulphide isomerase

Use

Tags

V5 tag

Expression

Mutation

C53S, C56S, C397S, C400S

Promoter

pCMV

Available since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-TRPT1

Plasmid

#184244

Purpose

For TRPT1 expression in mammalian cells

Depositor

Karla Feijs

Article

Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

Insert

TRPT1 (TRPT1 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-RalA(WT)

Plasmid

#224274

Purpose

Cell transfection and expression of RalA

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

RalA (RALA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LacR-TopBP1

Plasmid

#31317

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

FLAG and LacR

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-Flag-SREBP1

Plasmid

#134283

Purpose

Expresses Flag-tagged SREBP1 in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP1 (Srebf1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP1 isoform a precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1-Myc-RNF8

Plasmid

#221676

Purpose

Mammalian expression of Myc-tagged human RNF8

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (V155M)

Plasmid

#214161

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (V155M) (STING1 Human)

Use

Tags

Expression

Mammalian

Mutation

V155M

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3

Plasmid

#160250

Purpose

Express FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc TCF4

Plasmid

#16512

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-wt

Plasmid

#12965

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA (RHOA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nMagHigh1-EGFP-CAAX

Plasmid

#67300

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

nMagHigh1-EGFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MYC-Sumo3Q89P

Plasmid

#48963

Purpose

Expresses MYC-Sumo3Q90P mutant in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO3 (SUMO3 Human)

Use

Tags

MYC (EQKLISEED)

Expression

Mammalian

Mutation

Mutated Q89P

Promoter

T7

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-(empty)-TAG

Plasmid

#138209

Purpose

To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

Depositor

Neal Devaraj

Article

Busby et al Methods Enzymol. 2020;641:373-399. doi:

Type

Empty backbone

Use

T7 transcription

Tags

TAG sequence

Expression

Mammalian

Mutation

Promoter

CMV Promoter, T7 Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_Lambda-N-HA-Peptide

Plasmid

#92005

Purpose

Expresses Lambda-N-HA peptide

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Lambda-N-HA peptide

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Ufd1-Strep-HA

Plasmid

#113474

Purpose

Expression of human Ufd1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Ufd1 (UFD1 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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