Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,560 results for: chia

Showing: 3281 - 3320 of 5560 results

  1. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

    Plasmid
    #214950
    Purpose
    Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.
    Depositor
    Dan Mulvihill
    Article
    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1
    Inserts
    VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
    VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
    Use
    Tags
    6xHis, Amino half of venus BIFC fragment, Carboxy…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    214950-plasmid-map-sequence-id-439535

    Enlarge

  8. pKT-CNP-inact

    Plasmid
    #211804
    Purpose
    Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)
    Depositor
    Emily Weinert
    Article
    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.
    Insert
    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H73L H153L
    Promoter
    tet
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211804-plasmid-map-sequence-id-417773

    Enlarge

  18. pCAGGS-SMS1-TEV-HAT

    Plasmid
    #202557
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Use
    Tags
    Histidine-Affinity-tag (HAT) and TEV cleavable si…
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    202557-plasmid-map-sequence-id-399621

    Enlarge

  31. pBB0405-TEV-His12

    Plasmid
    #137040
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137040-plasmid-map-sequence-id-275693

    Enlarge

  32. pRevA-TEV-His12

    Plasmid
    #137034
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137034-plasmid-map-sequence-id-275692

    Enlarge

  33. pP13-TEV-His12

    Plasmid
    #137035
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137035-plasmid-map-sequence-id-275690

    Enlarge

  36. pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

    Plasmid
    #92315
    Purpose
    Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    B1 domain of protein L (PL) mutant K16TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70b (p70a promoter with one mutation)
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92315-plasmid-map-sequence-id-197049

    Enlarge

Showing: 3281 - 3320 of 5560 results