We narrowed to 24,482 results for: Spr;

1. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAEF5

   Plasmid

   #136305

   Purpose

   Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

   Depositor

   Gilles Fischer

   Article

   Agier et al Methods Mol Biol. 2021;2196:181-198. doi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB-CBEv1

   Plasmid

   #221138

   Purpose

   Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Promoter

   lacIq-Ptac

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ290

   Plasmid

   #129670

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Expression

   Plant

   Mutation

   AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB-Cas9-@GL1

   Plasmid

   #86779

   Purpose

   Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   sgRNA against GLABROUS1
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   Arabidopsis U6-26 promoter

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. SP6-hCas9-Ce-mRNA

   Plasmid

   #47911

   Purpose

   In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Insert

   hCas9 with C. elegans 5' and 3' UTRs
   

   Use

   CRISPR

   Promoter

   SP6

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1137=Hsp70Bb-Cas9[dsRed]

   Plasmid

   #149424

   Purpose

   The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Promoter

   Dmel Hsp70Bb

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLQ-KO-tgt50

   Plasmid

   #126578

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCasTns(Vch)-entry(BsaI)

   Plasmid

   #190273

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
   

   Expression

   Bacterial

   Mutation

   no

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ292

    Plasmid

    #129672

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LbCas12a

    Plasmid

    #188493

    Purpose

    Expresses FLAG tagged LbCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ714

    Plasmid

    #132955

    Purpose

    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    dFnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and nucleoplasmin NLS

    Promoter

    adh1

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDYSCKO-ADE1

    Plasmid

    #120264

    Purpose

    pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    pDYSCKO cassette-ADE1 targeting guide
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid

    #117070

    Purpose

    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

    Depositor

    Kim Failor

    Inserts

    Cas9
    SacB
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pADH139

    Plasmid

    #90987

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, C, mal LEU2 2 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ239A (dFnCas12a)-TV

    Plasmid

    #136379

    Purpose

    CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    dFnCas12a-TV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D917A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2-HybridBarcode

    Plasmid

    #174037

    Purpose

    For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    CRISPR target sites
    

    Use

    Tol2 transgenesis

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDYSCKO

    Plasmid

    #120263

    Purpose

    Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    DYSCKO cassette
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbdCas9S_P0526-sgRNA500

    Plasmid

    #149641

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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