We narrowed to 13,777 results for: ckb

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pEGFP-C1 Lifeact-EGFP-2XNLS

Plasmid

#58467

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Lifeact
2X NLS with 2X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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mKO2-N1

Plasmid

#54625

Purpose

Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Uac

Plasmid

#126959

Purpose

Universal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-DEST(r)

Plasmid

#190238

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-MCS-3AID-HA-2A-mCherryBSD

Plasmid

#225700

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mCherry

Expression

Mammalian

Promoter

pEF1A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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hAAVS1 TALEN Left

Plasmid

#52341

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Left

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

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hAAVS1 TALEN Right

Plasmid

#52342

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Right

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

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epi-BE4max

Plasmid

#135975

Purpose

Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-N1

Plasmid

#54620

Purpose

Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Type

Empty backbone

Tags

mIFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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SlugCas9

Plasmid

#163793

Purpose

Expresses SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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mCardinal-C1

Plasmid

#54799

Purpose

Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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FusionRed-pBAD

Plasmid

#54677

Purpose

Localization: Protein Expression Vector , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Tags

6xHis and FusionRed

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TR-Csy4-H29A

Plasmid

#80602

Purpose

Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-H29A

Use

AAV

Expression

Mammalian

Mutation

His29 mutated to Ala

Promoter

CBA

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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epi-BE4max-NG

Plasmid

#135977

Purpose

Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
PL452-hGH-polyA-PGK-neoR

Plasmid

#191681

Purpose

Donor plasmid backbone PGK-neoR

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Type

Empty backbone

Expression

Mammalian

Mutation

WT

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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mEos4b-C1

Plasmid

#54812

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4b

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pJEP207-AAV-CMV-MCS-pA

Plasmid

#78318

Purpose

AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pVH1002-Tier2(pUC ori)

Plasmid

#169600

Purpose

Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (no Cre)

Plasmid

#200370

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro

Plasmid

#99636

Purpose

Empty backbone, 3rd generation lentiviral vector

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

SFFVp

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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