166,289 results

1. TFORF1932

   Plasmid

   #142292

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUN (JUN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTn7-SCOUT11.Y

   Plasmid

   #201004

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   sYFP2
   

   Expression

   Bacterial

   Promoter

   J23100

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK3079

   Plasmid

   #78741

   Purpose

   Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   AsCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCK054 pDEST exorh:mCerulean

   Plasmid

   #195985

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24b-APEX2

   Plasmid

   #111702

   Purpose

   For IPTG-inducible expression of APEX2 in E. coli

   Depositor

   Jessica Seeliger

   Article

   Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

   Insert

   APEX2
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH136

   Plasmid

   #47554

   Purpose

   pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

   Insert

   URA3
   

   Use

   Pcr template

   Promoter

   Native URA3 promoter, -243 to -1.

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSK664

   Plasmid

   #205130

   Purpose

   Expression of GFP-2xPX (from SnxA) in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   tandem 2xPX domain of SnxA
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Neo-Bam

   Plasmid

   #16440

   Purpose

   Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEST-p15A-PLtetO1-tetR-T500

   Plasmid

   #45391

   Depositor

   Vincent Noireaux

   Article

   Shin et al J Biol Eng. 2010 Jun 24;4:8.

   Insert

   tet repressor
   

   Expression

   Bacterial

   Promoter

   PL-tetO1

   Available Since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACU2

    Plasmid

    #31223

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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