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PX394 Neisseria cinerea Cas9
Plasmid#68331PurposeNeisseria cinerea Cas9DepositorInsertNcCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
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dCas9-3×RFP-P2A-CASANOVA/gRNA telo
Plasmid#137711PurposeVector co-expressing dCas9-3×RFP-P2A-CASANOVA and sgRNA teloDepositorInsertdCas9-3×RFP-P2A-CASANOVA/gRNA telo
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
Plasmid#137712PurposeVector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA teloDepositorInsertdCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRSET.HaloTag.v7.CoOp.Cys-less
Plasmid#195487PurposeBacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.DepositorInsertHaloTag.v7
UseTags6xHISExpressionBacterialMutationC61T, C262VPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (KmR)
Plasmid#177209PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK GFP Paxillin Y31A
Plasmid#50530DepositorInsertPaxillin (PXN Human)
UseTagsGFPExpressionMammalianMutationY31APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-B.1.1.7-FLAG
Plasmid#177093PurposeB.1.1.7 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein B.1.1.7 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAc5.1B-EGFP-DmDis3
Plasmid#66592Purposeexpresses EGFP-tagged Dis3 in Drosophila cellsDepositorInsertDIS3 (Dis3 Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterActin 5CAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX406 Pasteurella multocida Cas9
Plasmid#68703PurposePasteurella multocida Cas9DepositorInsertPmCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
mT2A-td-sfGFP-FNF
Plasmid#182339PurposeRecombineering cassette plasmid for T2A-td-sfGFP-FNFDepositorInsertT2A-td-sfGFP-FNF
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)
Plasmid#48286DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK551
Plasmid#72425PurposeProduces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase, N-terminal His6 tag (H6AaUctD)DepositorInsertputative CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RB-PE2
Plasmid#173903PurposeSB-transposon with inducible expression of SpCas9 PE2DepositorInsertPE2
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJK406
Plasmid#72420PurposeProduces Acetobacter aceti 1023 Est05, C-terminal His6 tagDepositorInserthypothetical protein
UseTagsHis6ExpressionBacterialMutationadditional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…PromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/Flag-mKO2
Plasmid#110371PurposeMammalian expression of mKO2 with FLAG tag, Flp-In T-Rex systemDepositorInsertmonomeric Kusabira Orange 2
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIC65
Plasmid#63061PurposeRetroviral expression of EGFP-TEV-S-tag Mis12 for protein localization and affinity purification (LAP)DepositorInserthMis12, IMAGE clone 3351245 (MIS12 Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationL68RPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
TC9nickpAB
Plasmid#71311Purposeused as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsIDepositorInsertCas9 nickase
UseIn vitro transcriptionTagsNLSExpressionMutationD10A nickasePromoterT7Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBS L30 mRuby3-Rab5c
Plasmid#166426PurposeRab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab5C
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HLA-B15-His
Plasmid#104837PurposeHLA-B150101 cloned as a His tagDepositorInsertmajor histocompatibility complex, class I, B (HLA-B Human)
UseEntry vector for gateway systemTagshisExpressionMutation2 differences compared to original B15: L2->R …PromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMIY-Arg1-Flag
Plasmid#34581DepositorInsertArg1 (Arg1 Mouse)
UseRetroviralTagsFLAG and IRES-YFPExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
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PX390 Flavobacterium columnare Cas9
Plasmid#68327PurposeFlavobacterium columnare Cas9DepositorInsertFcCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
UseTagsFLAG tagExpressionYeastMutationPromoterGAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
ROR2
Plasmid#39183PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertROR2 (ROR2 Human)
UseBaculovirus expressionTagsHis TevExpressionMutationPromoterpolyhedrinAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV expression vector with BioBrick polypromoter restriction sites (14-T)
Plasmid#48314DepositorTypeEmpty backboneUseTagsHis6 and ThioredoxinExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pET His6 Mocr TEV expression vector with BioBrick polypromoter restriction sites (14-O)
Plasmid#48310DepositorTypeEmpty backboneUseTagsHis6 and MocrExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pLenti4/V5-DEST-P85(G376R)
Plasmid#40220DepositorInsertP85(G376R) (PIK3R1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationchanged Glycine 376 to ArgininePromoterCMVAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only
