We narrowed to 62,913 results for: cloning
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Plasmid#153209Purposeexpress CARPID BASU-dCasRx fusion protein in mammalian cellsDepositorInsertBASU-dCasRx
UseCRISPR and LentiviralExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR207 SARS-CoV-2 ORF3A
Plasmid#141271PurposeGateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLGB13
Plasmid#126618PurposeSuicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCasKP-apr
Plasmid#117231PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
TagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-Ftractin-mCherry
Plasmid#85131Purposelentiviral expression of an F-actin live-cell marker (mCherry)DepositorInsertFtractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
UseLentiviralTagsmCherryPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLemir9-124
Plasmid#31779DepositorAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Mm.cargo(Cre)
Plasmid#174862PurposeExpresses mouse Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by MmPeg10 UTRs
ExpressionMammalianPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti
Plasmid#135955PurposeNeuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) N-terminal
UseLentiviralExpressionMammalianPromoterhSynapsinAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C1(1-29)-miniTurbo-V5_pLX304
Plasmid#107176Purposeexpresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vectorDepositorInsertminiTurbo (BirA mutant)
UseLentiviralMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-MS2-Tet1-CD
Plasmid#83341PurposeTo introduce MS2 coat protein fused Tet1-CDDepositorInsertMS2-Tet1-CD
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST51-LRRK2-WT
Plasmid#25080DepositorPublicationAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLAG/HA-MOV10
Plasmid#10976DepositorAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
TagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-SRRM2-mCherry
Plasmid#174089PurposeInducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on systemDepositorAvailable sinceSept. 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-AsCas12f1
Plasmid#171614PurposeAsCas12f1-based Human cell genome editingDepositorInsertAsCas12f1 and sgRNA_v1
ExpressionMammalianAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSCAR_Cas9-blast_GFP
Plasmid#162074Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistanceDepositorInsertsCas9-2a-blasticidin S deaminase
EGFP
UseCRISPR, Cre/Lox, and LentiviralExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL1765 (pSPIN-R, R*MmeI)
Plasmid#160734PurposeSingle-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6.2-Nanoluc-ccdB
Plasmid#87078PurposeLentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by