We narrowed to 6,946 results for: pcDNA3.1+
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Plasmid#73209PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertkappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DDX41-GFP
Plasmid#175494PurposeTotal RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDX41 (DDX41 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1Xpress-Bcr-HisB
Plasmid#32508DepositorAvailable sinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertALIX (PDCD6IP Synthetic, Human)
Tags6xHIS and mNeonGreenExpressionMammalianPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_YB TATA-sfGFP-CMV_dsRed
Plasmid#117399PurposesfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSuperFolder GFP
ExpressionMammalianAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS9
Plasmid#111765PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 moduleDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FlagETR3 (CELF2)
Plasmid#96900Purposeexpression of Flag-tagged human CELF2 (aka CUGBP2)DepositorAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-PhoCl-mCherry
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cre Shine
Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseCre/LoxTagseGFPExpressionMammalianPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EYFP-Clathrin
Plasmid#20921DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClathrin, light polypeptide (Lca) (Clta Mouse)
TagsEYFPExpressionMammalianAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRUPATH Triple Gai1
Plasmid#196048PurposeEncodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-sACE2-WT(732)-8h
Plasmid#154101PurposeMammalian expression plasmid for dimeric soluble ACE2 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PCDNA3-GFP1-9 T2A mCherry
Plasmid#124430PurposeExpresses GFP1-9 and T2A mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP1-9 T2A mCherry
ExpressionMammalianAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
negMaLionG
Plasmid#113907Purposenegative control for Green ATP sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnegMaLionG
ExpressionMammalianAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CCR5
Plasmid#98943PurposeMammalian expression plasmid for untagged human CCR5DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCR5 (CCR5 Human)
ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832AAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-ATBF1
Plasmid#40927DepositorInsertATBF1 (ZFHX3 Human)
TagsFLAGExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)
Plasmid#47387Purposeallow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectinDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertglutamate-gated chloride Channels
ExpressionMammalianMutationLeu9 prime mutated to F (Phenylalanine in M3-M4 l…PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by