We narrowed to 16,820 results for: URE

1. pGP-CMV-GCaMP6m

   Plasmid

   #40754

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6m
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+_FH-AGO2-WT

   Plasmid

   #92006

   Purpose

   Expresses FLAG-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-SH3(2)-5R

   Plasmid

   #112089

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-5R (NCK1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only repeats of the second SH3…

   Promoter

   Lac

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. DYRK1A/pTRE3G-FL-hXIST

   Plasmid

   #149608

   Purpose

   Express doxycycline-inducible full length of human XIST cDNA

   Depositor

   Jeanne Lawrence

   Article

   Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

   Insert

   X-inactive specific transcript (XIST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE3G

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7C-HSP90AA1

   Plasmid

   #177658

   Purpose

   Recombinant protein synthesis of human HSP90AA1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSP90AA1 (HSP90AA1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. HR180-LGR5-GFP

   Plasmid

   #129092

   Purpose

   KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

   Depositor

   Toshiro Sato

   Article

   Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

   Insert

   EGFP and LGR5 homology arms (LGR5 Human)
   

   Use

   Homologous recombination donor plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1_MeCP2(WT)

   Plasmid

   #110186

   Purpose

   Expression of mouse MeCP2 (WT) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG.LSL.tdTomato

   Plasmid

   #100048

   Purpose

   AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-ABE7.9

   Plasmid

   #102918

   Purpose

   expresses ABE7.9 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.9
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. HR180-LGR5-CreER

    Plasmid

    #129093

    Purpose

    KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    CreERT2 and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TET-mcMyc

    Plasmid

    #20888

    Purpose

    piggyBac vector with tet inducible mcMyc

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    cMyc (Myc Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSI-Target-AID-NG

    Plasmid

    #119861

    Purpose

    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-EglN2-pLenti6

    Plasmid

    #36950

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    EglN2 (EGLN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-EglN3-pLenti6

    Plasmid

    #36951

    Depositor

    William Kaelin

    Article

    Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

    Insert

    EglN3 (EGLN3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. Arrb1-RFP

    Plasmid

    #35401

    Depositor

    Robert Lefkowitz

    Article

    Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

    Insert

    beta-arrestin-1 (Arrb1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

    Plasmid

    #35501

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR1a-tagRFP-KDEL

    Plasmid

    #114177

    Purpose

    Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Gateway entry clone

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-ABE6.3

    Plasmid

    #102916

    Purpose

    expresses ABE6.3 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE6.3
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CD99-CD4d3+4-bio

    Plasmid

    #73097

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding CD99 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    CD99 (CD99 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-ABE7.8

    Plasmid

    #102917

    Purpose

    expresses ABE7.8 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.8
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CR1-isoformB-CD4d3+4-bio

    Plasmid

    #73099

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding CR1-isoformB with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    CR1 isoform B (CR1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Human a3 nicotinic receptor subunit EM construct

    Plasmid

    #163960

    Purpose

    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct

    Depositor

    Ryan Hibbs

    Article

    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

    Insert

    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    apocytochrome b(562)RIL (BRIL)

    Expression

    Mammalian

    Mutation

    Asn348-Ser402 replaced with apocytochrome b(562)R…

    Promoter

    CMV

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMSCV-SMYD3

    Plasmid

    #85650

    Purpose

    retroviral expression of human SMYD3

    Depositor

    Julien Sage

    Article

    Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

    Insert

    SMYD3 (SMYD3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. CD47-CD4d3+4-bio

    Plasmid

    #73121

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding CD47 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    CD47 antigen (CD47 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-TET-mOct4

    Plasmid

    #20907

    Purpose

    piggyBac vector with tet inducible mOct4

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSUMO His6 SUMO PYL1 (36-211)

    Plasmid

    #177843

    Purpose

    Bacterial Expression of PYL1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 1 (PYL1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMIR-21 Reporter

    Plasmid

    #71870

    Purpose

    Mammalian expression vector for the analysis of miR-21 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-21
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR4-EGFP_opt

    Plasmid

    #106334

    Purpose

    Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    codon optimized for gene synthesis without affect…

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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