We narrowed to 22,773 results for: crispr

1. pColE1_sgRNA_pos5_AmpR

   Plasmid

   #119148

   Purpose

   Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 5 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pColE1_sgRNA_pos2_AmpR

   Plasmid

   #119145

   Purpose

   Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 2 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB8622

   Plasmid

   #126912

   Purpose

   Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7mitfagRNA

   Plasmid

   #47931

   Purpose

   in vitro transcription of mitfa gRNA

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   mitfa gRNA (mitfa Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA with rpr-1 promoter

   Plasmid

   #48961

   Purpose

   to drive the sgRNA expression under RNase P non-coding RNA promoter

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rpr-1 promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6>Ebf.774(F+E)

   Plasmid

   #59990

   Purpose

   U6 promoter driving Ebf.774 (F+E) sgRNA

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   Ebf.774 (F+E) sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ciinte.U6

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAN-AND

   Plasmid

   #62308

   Purpose

   synthetic circuit: AND gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Inserts

   A1NT sgRNA
   A4NT sgRNA
   A2NT sgRNA
   A1NT
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PA2, PPhlF, pA4, and pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7ddx19gRNA

   Plasmid

   #47932

   Purpose

   in vitro transcription of ddx19 gRNA

   Depositor

   Wenbiao Chen, Susan Wente

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   ddx19 gRNA (ddx19 Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. psBIG1a ActBT2ATetP2AP - mAGH

   Plasmid

   #229973

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - mAGH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. psBIG1a ActBT2ATetP2AP - H

    Plasmid

    #229972

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. psBIG1a ActBT2AmcherryP2AP -TagBFPH

    Plasmid

    #229971

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2AmcherryP2AP -TagBFPH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-dSa VP64 Afp

    Plasmid

    #99697

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnCas9-AID

    Plasmid

    #196242

    Purpose

    Plasmid expressing the nCas9-AID fusion

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    nickase Cas9 fused to AID
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBK45 pHomL-RPL18Bp-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179029

    Purpose

    Parent vector for markerless yeast integration cassettes with untagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBBK44 pHomL-TEF2p-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179028

    Purpose

    Parent vector for markerless yeast integration cassettes with untagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAN-PBAD-sgRNA-A1NT

    Plasmid

    #62255

    Purpose

    expression of A1NT sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A1NT
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJKW0458

    Plasmid

    #107587

    Purpose

    p35S:CAS9

    Depositor

    Jing-Ke Weng

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35S

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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