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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,947 results for: mcs

Showing: 1681 - 1700 of 4947 results

  1. NK811

    Plasmid
    #196408
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Mutation
    N/A (synthetic)
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196408-plasmid-map-sequence-id-384995

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  2. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201533-plasmid-map-sequence-id-398133

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  3. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201535-plasmid-map-sequence-id-398128

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  8. pOP004

    Plasmid
    #185985
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Expression
    Bacterial
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185985-plasmid-map-sequence-id-378299

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  9. pOP005

    Plasmid
    #186030
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Expression
    Bacterial
    Available Since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186030-plasmid-map-sequence-id-371540

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  16. pJB03

    Plasmid
    #111961
    Purpose
    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    Tac
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111961-plasmid-map-sequence-id-219904

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  17. pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H

    Plasmid
    #108378
    Purpose
    FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108378-plasmid-map-sequence-id-209430

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  18. pcDNA5/FRT/TO-TPI_WT-xrRNA-4H

    Plasmid
    #108377
    Purpose
    FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108377-plasmid-map-sequence-id-209424

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  19. p5A1-T

    Plasmid
    #92017
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available Since
    Jan. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    92017-plasmid-map-sequence-id-201852

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  20. p5C3-T

    Plasmid
    #92022
    Purpose
    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92022-plasmid-map-sequence-id-190017

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Showing: 1681 - 1700 of 4947 results