4,180 results

1. pSIJ8

   Plasmid

   #68122

   Purpose

   Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

   Depositor

   Alex Nielsen

   Article

   Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

   Inserts

   flp recombinase
   rhaS
   rhaR
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   Dec. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. His6-MBP-uTEV3

   Plasmid

   #135464

   Purpose

   Bacterial expression of uTEV3 (full-length) protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   His6-MBP-uTEV3
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation (improves stability) , I138T/S153N…

   Promoter

   T5

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HA-p62

   Plasmid

   #28027

   Purpose

   Mammalian expression of HA tagged human p62

   Depositor

   Qing Zhong

   Article

   Fan et al Autophagy. 2010 Jul 22. 6(5):.

   Insert

   p62 (SQSTM1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-D12L

   Plasmid

   #89161

   Purpose

   Express vaccinia virus D12L in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D12L
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBtet-Pur

   Plasmid

   #60507

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-MCS2

   Plasmid

   #46954

   Purpose

   Cloning plasmid for the generation of Knock-In in human cells using rAAV.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-CMV-LoxP-DsRed-LoxP-eGFP

   Plasmid

   #65726

   Purpose

   Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

   Depositor

   Jacco van Rheenen

   Article

   Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

   Insert

   LoxP-DsRed-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag-TRIM28

   Plasmid

   #124960

   Purpose

   Mammalian expression of Human TRIM28

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge