164,577 results

1. CROP-seq-opti

   Plasmid

   #106280

   Purpose

   A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pROSA26-PA

   Plasmid

   #21271

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2CG2-U6:gRNA

   Plasmid

   #63156

   Purpose

   Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDx_mScarlet-I3

   Plasmid

   #189757

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLminP_Luc2P_RE5

   Plasmid

   #90339

   Purpose

   STAT3 - Stat3 homodimer gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   STAT3

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

   Plasmid

   #67977

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_120

   Plasmid

   #96917

   Purpose

   for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

   Depositor

   John Doench, David Root

   Article

   Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX307-CRBN-3F-BirA

   Plasmid

   #220146

   Purpose

   Expresses CRBN-BirA for CRBN substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   Cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

    Plasmid

    #188384

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-3XHA-EGFP-OMP25

    Plasmid

    #83356

    Purpose

    For tagging mitochondria with HA epitopes (HA-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XHA-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHMattR3-attR4-GFP

    Plasmid

    #196247

    Purpose

    pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV4-ApoE4

    Plasmid

    #87087

    Purpose

    Expresses ApoE4 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E4 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 8xGTIIC-luciferase

    Plasmid

    #34615

    Purpose

    YAP/TAZ-responsive synthetic promoter driving luciferase expression

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

    Insert

    synthetic TEAD luciferase reporter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. emiRFP670-cGAS

    Plasmid

    #231896

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-PHP.S

    Plasmid

    #103006

    Purpose

    non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.S VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-Cre

    Plasmid

    #198712

    Purpose

    For lentiviral expression of Cre recombinase in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Insert

    Pgk-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-rab7 WT

    Plasmid

    #12605

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-mTagBFP2

    Plasmid

    #122373

    Purpose

    Expresses mTagBFP2 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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