172,049 results

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

ADAR1p150 (ADAR Human)

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET23b-NTF2

Plasmid

#108918

Purpose

Expresses NTF2

Depositor

Larry Gerace

Article

Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

Insert

NTF2

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMOS007: PercevalHR ATP/ADP sensor (cytosolic)

Plasmid

#163061

Purpose

PercevalHR ATP/ADP sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Promoter

NF-kappaB binding site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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OpenCRISPR-1 sgRNA-16nt stem

Plasmid

#221566

Purpose

Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 16nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-Akt-FoxO3a-KTR-EGFP

Plasmid

#125131

Purpose

Fluorescent reporter for Akt activity

Depositor

Kazuhiro Aoki

Article

Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

Insert

FOXO2 (FOXO3 Human)

Tags

Enhanced green fluorescent protein (EGFP)

Expression

Mammalian

Mutation

FoxO3A 1-402 amino acid

Promoter

CAG promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV LIC cloning vector (1M)

Plasmid

#29656

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pMaster12

Plasmid

#58527

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
Myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
NANOG (NANOG Human)
IRES
NR5A2 (NR5A2 Human)
miR302/367 (MIR367, MIR302A Human)

Expression

Mammalian

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSynSRE-T-Luc

Plasmid

#60444

Purpose

Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

Depositor

Timothy Osborne

Article

Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

Insert

HMG-CoA synthase promoter (-324/-225)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (Blast)

Plasmid

#80921

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NlucP

Plasmid

#162595

Purpose

C-terminally PEST-tagged NanoLuc under TRE3G promoter

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

NanoLuc-PEST

Tags

PEST

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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ilux pGEX(-)

Plasmid

#107879

Purpose

Bacterial expression of ilux for bioluminescence imaging

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

Insert

ilux

Expression

Bacterial

Promoter

tac

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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MSCVneo-HA-ER-Hoxb8

Plasmid

#222291

Purpose

The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP

Plasmid

#37825

Purpose

AAV-mediated expression of GFP under the CAG promoter

Depositor

Edward Boyden

Has Service

AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti PGK V5-LUC Neo (w623-2)

Plasmid

#21471

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

V5-LUC

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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