We narrowed to 9,280 results for: Pol

Showing: 4251 - 4300 of 9280 results

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WasCFP /pQE-30

Plasmid

#79858

Purpose

Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

Insert

WasCFP

Tags

HisTag

Expression

Bacterial

Mutation

Cyan fluorescent protein mCerulean with substitut…

Promoter

T5 promoter / lac operator

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas9-162A

Plasmid

#84918

Purpose

for in vitro transcription of Cas9 with polyA tail encoded

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Cas9

Tags

Flag, NLS, and polyA tail

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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p416 25Q GPD

Plasmid

#1177

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-GFP-Tdtomato-iRFP670

Plasmid

#111812

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
Tdtomato
iRFP670

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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ThcCut1_pET29b

Plasmid

#215429

Purpose

Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ThcCut1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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p416 103Q GPD

Plasmid

#1180

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
EF1m-ELP

Plasmid

#170763

Purpose

EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

EF1m-ELP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Ki67∆Nterm-mNeonGreen-IRESpuro2

Plasmid

#183740

Purpose

Expresses Ki67∆Nterm-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain + all Ki-67 repeats (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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UMG-SP-3_pET29b

Plasmid

#215435

Purpose

Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-3

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-MLC2 WT

Plasmid

#195235

Purpose

Mammalian expression of GFP tagged MLC2. Wild-type

Depositor

David Bryant, Keith Mostov

Article

Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

Insert

MLC2 (MYL2 Human)

Expression

Mammalian

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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GFAP-Arclight

Plasmid

#187978

Purpose

Expresses the fluorescent voltage indicator Arclight under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Arclight A242

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_2024_F

Plasmid

#237360

Purpose

Expression plasmid for RSV B F from 2024

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F from 2024 human codon optimized from Genbank Accesion PP660445

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_1992_F

Plasmid

#237358

Purpose

Expression plasmid for RSV B F from 1992

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F from 1992 human codon optimized from Genbank Accesion OK649748

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_OR795252_F_N201S

Plasmid

#237363

Purpose

Expression plasmid for RSV B F from OR795252

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F human codon optimized from Genbank Accesion OR79525

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_1982_F

Plasmid

#237357

Purpose

Expression plasmid for RSV B F from 1982

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F from 1982 human codon optimized from Genbank Accesion MG642043

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_2019_F

Plasmid

#237359

Purpose

Expression plasmid for RSV B F from 2019

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F from 2019 human codon optimized from Genbank Accesion PP352433

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_MN365362_F_K68Q

Plasmid

#237361

Purpose

Expression plasmid for RSV B F from MN365362

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F human codon optimized from Genbank Accesion MN36536

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_OQ279862_F_N201T

Plasmid

#237362

Purpose

Expression plasmid for RSV B F from OQ279862

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV B F human codon optimized from Genbank Accesion OQ27986

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUAP4

Plasmid

#136079

Purpose

Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-myc-Ataxin3Q84

Plasmid

#22124

Depositor

Henry Paulson

Article

Chai et al J Neurosci. 1999 Dec 1. 19(23):10338-47.

Insert

Ataxin 3 (ATXN3 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense

Plasmid

#46834

Purpose

Express cGFP transcript ending in the wildtype MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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Myc-CICf

Plasmid

#48185

Purpose

Expresses Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pEx-insertion-erm

Plasmid

#172210

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-mCherry-Ki67

Plasmid

#183750

Purpose

Expresses EGFP-mCherry-Ki67

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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AL12R-HSP101

Plasmid

#161014

Purpose

PP7-tagged reporter driven by the Arabidopsis HSP101 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEx-insertion-erm-T1T2

Plasmid

#172212

Purpose

For genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminator

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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p416 72Q GPD

Plasmid

#1179

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 72Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pmCherry-C1-pmDH

Plasmid

#67897

Purpose

Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-pmDH

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

Plasmid

#183741

Purpose

Expresses Ki67 (8 repeats+LR)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67 LR domain + 8 Ki-67 repeats

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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3TERM_Nos-35S

Plasmid

#136116

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos-35S (A. tumefaciens, CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
p416 103Q GAL

Plasmid

#1186

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1 beta-catenin Y654F

Plasmid

#16072

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

Y654F, *(see below)

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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NylCA_pET28a

Plasmid

#215418

Purpose

Expression construct for NylCA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NylCp2_pET28a

Plasmid

#215420

Purpose

Expression construct for NylCp2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCk3_spacer

Plasmid

#136073

Purpose

Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pYC3.60-C1

Plasmid

#67899

Purpose

Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

YC3.60

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ConVERGD-N2cG-WPRE

Plasmid

#218757

Purpose

Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

N2cG

Use

AAV

Mutation

See Depositor Comments

Promoter

hSyn

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV PIG IMP-1-short

Plasmid

#21659

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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FU-MAP2-Gateway

Plasmid

#43915

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5 and non-standard His tag (HHRHHH)

Expression

Mammalian

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-NEMO

Plasmid

#11965

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

GST

Expression

Bacterial

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFlag-Dam

Plasmid

#121909

Purpose

Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Dam human codon optimized (dam E. coli)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AfNitB_pET29b

Plasmid

#215401

Purpose

Expression construct for AfNitB gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

AfNitB

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pK-myc-Par6C

Plasmid

#15474

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6C (PARD6A Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mVenus-MKL2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 WAVE 2

Plasmid

#67098

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WAVE 2 (WASF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pZY102

Plasmid

#73933

Purpose

This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites. This plasmid is recommended for use as empty control for service event request.

Depositor

Zhanyuan Zhang

Article

Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E

Inserts

bar
GUS

Expression

Plant

Mutation

intron is inserted into the GUS coding region

Promoter

35S

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 9,280 results for: Pol