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VGAT scFv [L118/14]

Plasmid

#206754

Purpose

Mammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Bmi1-overexpression

Plasmid

#21577

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Tags

ires-eGFP

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRB

Plasmid

#131036

Purpose

Expresses OT-I TCRB

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Ezh2-IRES-GFP

Plasmid

#107146

Purpose

Mammalian expression of mouse Ezh2

Depositor

Martine Roussel

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-puro-N-3HA-SREBP2-C-Flag

Plasmid

#134287

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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Col1A2:TFPnls-T2a-mErt-Cre-Ert

Plasmid

#111151

Purpose

Fibroblasts specific inducible Cre

Depositor

Elly Tanaka

Insert

TFPnls-T2a-mErt-Cre-Ert

Use

Cre/Lox

Mutation

wt

Promoter

Col1A2

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPten#2/Cre

Plasmid

#173646

Purpose

Expresses a Pten-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pten (Pten Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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NL4-3Δ6-drEGFP-IRES-mThy1.2

Plasmid

#207881

Purpose

HIV reporter vector expressing dGFP and mThy1.2 from Env locus

Depositor

Edward Browne

Article

Peterson et al Cell Chem Biol. 2023 Dec 21;30(12):1617-

Insert

destabilized EGFP-IRES-mThy1.2

Use

Lentiviral

Promoter

5' lentiviral LTR

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGluSnFR3.v857.SGZ

Plasmid

#178337

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE puro PPAR gamma 2

Plasmid

#8859

Depositor

Bruce Spiegelman

Article

Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

Insert

PPAR gamma (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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CMV-MyoD

Plasmid

#8398

Depositor

Andrew Lassar

Article

Skapek et al Mol Cell Biol 1996 Dec;16(12):7043-53.

Insert

MyoD (Myod1 Mouse)

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-ZnF-hSyn-DIO-PSD95FingR-mGreenLantern

Plasmid

#200278

Purpose

Genetically encoded fluorescent probe for labelling endogenous excitatory synapses in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

ZnF-FingR-PSD95-mGreenLantern

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Promoter

human Synapsin

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Nurr1

Plasmid

#35000

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Nuclear receptor subfamily 4 group A member 2 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-ZnF-hSyn-DIO-GPHNFingR-eGFP

Plasmid

#200279

Purpose

Genetically encoded fluorescent probe for labelling endogenous inhibitory synapses in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

zFn-FingR-GPHN-eGFP

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

human Synapsin

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-NICD

Plasmid

#26891

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD (Notch1 Mouse)

Tags

FLAG and b-globin polyA

Expression

Mammalian

Mutation

E2299K

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
tetO-Hand2

Plasmid

#46028

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Hand2 (Hand2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRF3

Plasmid

#221260

Purpose

Expression of IRF3 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRF3 (Irf3 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

Plasmid

#205281

Purpose

CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.

Depositor

Matthias Prigge

Insert

GCaMP6s

Use

AAV

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1(pCMV5)

Plasmid

#12148

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 (Foxo1 Mouse)

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

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mTLR4 flag

Plasmid

#13087

Depositor

Ruslan Medzhitov

Insert

TLR4 (Tlr4 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-19 have been replaced with mammalia…

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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