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Plasmid#19346DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P3nmt1-3FLAG
Plasmid#19345DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOSV802
Plasmid#126595Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P81nmt1-3FLAG
Plasmid#19347DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOSV807
Plasmid#126600Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV811
Plasmid#126604Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-Purg1-3FLAG
Plasmid#19355DepositorInserturg1 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOSV804
Plasmid#126597Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV812
Plasmid#126605Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV808
Plasmid#126601Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV810
Plasmid#126603Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV806
Plasmid#126606Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV803
Plasmid#126596Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCX-HO1-2A-EGFP
Plasmid#74672PurposeMammalian expression of HO1 and EGFPDepositorInsertHO1 (HMOX1 Human)
UseTags2A-EGFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP-VPR
Plasmid#181907PurposeFluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-RtaDepositorInsertdCas9-VPR
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc IRES neo
Plasmid#98294PurposeFor constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance geneDepositorInsertsFirefly luciferase
IRES
Neomycin resistance gene (NeoR)
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMECP2-GFP
Plasmid#181904PurposeFluorescently tagged MECP2, GFPDepositorInsertMECP2 (Mecp2 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMECP2-RFP
Plasmid#181905PurposeFluorescently tagged MECP2, RFPDepositorInsertMECP2 (Mecp2 Mouse)
UseTagsRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HS_NAM-B1
Plasmid#154064PurposeLevel M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosTDepositorInsertsHygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterHvHSP17, OsAct, and ZmUbiAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-YAP1 CA
Plasmid#79502PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDSDepositorInsertYAP1 (YAP1 Human)
UseMultisite gateway entry cloneTags3XFLAGExpressionMutationS127APromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP249 pGK-dCas9-DNMT3a V3
Plasmid#100938PurposedCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1aDepositorInsertdCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
UseCRISPR and LentiviralTags3xHA and 3xTy1ExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hNCL
Plasmid#181886PurposeSoluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hNCL (NCL Mustard Weed, Human)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-hphMX6
Plasmid#19342DepositorInserthphMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-bleMX6
Plasmid#19344DepositorInsertbleMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
POC1551
Plasmid#226498PurposepMOBK360_StandVKL: Standard Vector (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protectionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB2H2*
Plasmid#163834PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)DepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationTCPTP R222MPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB2H12*
Plasmid#163838PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12 (Y64A)DepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationPTPN12 Y64APromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB2H6*
Plasmid#163836PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1 (R459M)DepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationSHP1 R459MPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB2Hopt*
Plasmid#163831PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B(C215S)DepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationPTP1B C215SPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB2HoptX
Plasmid#163832PurposeInactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1BDepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationMidT Substrate Y4FPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPL5617_pUG_PuroR
Plasmid#60928PurposePCR template vector for generating puromycin resistance cassette.DepositorInsertpuromycin N-Acetyltransferase
UseLoxp flanked deletion cassette templateTagsExpressionMutationPromoterTEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG_hPPP1R15B_4-713_mCherry_UK1298
Plasmid#80707Purposemammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)DepositorInserthuman PPP1R15B (PPP1R15B Human)
UseTagsmCherryExpressionMammalianMutationCDS 4-713 no stop codonPromoterCMVAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CAAX PuroR
Plasmid#166228PurposeLentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membraneDepositorInsertmCherry-CAAX
UseLentiviralTagsCAAXExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD-IFP2.0C
Plasmid#215750PurposeExpresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(WT)-FLAG
Plasmid#182654PurposeExpresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertwild type mouse neurofilament light chain (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-eDHFR-MCS
Plasmid#172108PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFRDepositorInsertEGFP-eDHFR-20aa (folA E. coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
rat APOBEC1-EGFP
Plasmid#112857Purposeplasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion proteinDepositorInsertapolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP-IFP2.0C
Plasmid#215752PurposeExpresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xCBX7CD-IFP2.0C
Plasmid#215754PurposeExpresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensorsDepositorInsert2xCBX7CD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC12
Plasmid#205068PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 12 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC16
Plasmid#205069PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 16 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC18
Plasmid#205070PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 18 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC20
Plasmid#205071PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 20 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC9
Plasmid#205067PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 9 amino acidsPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo W42A
Plasmid#191399PurposeW42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo W42A
UseTags3xFLAGExpressionMammalianMutationW42APromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTurboRFP-Farnesyl-5
Plasmid#58057PurposeLocalization: Plasma Membrane, Excitation: 553, Emission: 576DepositorInsertFarnesyl (HRAS Human)
UseTagstdTurboRFPExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cwc25-pRSETA
Plasmid#61043PurposeEncodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.DepositorInsertCwc25
UseTagsTEV site, Histidine TagExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only