We narrowed to 122,562 results for: CIL
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Plasmid#122545PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBT3043
Plasmid#122546PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-EF1a-D614G-N501Y
Plasmid#177939PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.DepositorAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9_ctrl
Plasmid#100981PurposeExpresses ThermoCas9 and its sgRNA moduleDepositorInsertsCas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialPromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfrbeta-EGFPN
Plasmid#66790PurposeExpression of murine PDGFRbeta tagged with GFP under the control of EF1a promoterDepositorAvailable SinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa1 WT
Plasmid#69824PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseLentiviralTagsHAExpressionMammalianPromoterminimal CMV promoter with tetracycline operatorAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)
Plasmid#133794PurposeU6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018DepositorInsertNmeCas9 and Nme2Cas9 sgRNA entry vector
ExpressionMammalianMutationsgRNA architecture from Amrani et al. Genome Biol…PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2
Plasmid#86237PurposeLentivirus for expression of human Plexin-B2DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralExpressionMammalianMutationfull length PLXNB2, CRISPR resistantPromoterhuman PGK promoterAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p23-puro-EGFP-RPA194
Plasmid#179237Purposeoverexpression in human cellsDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
ExpressionBacterialPromoterPcel and trcAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab11-DN (S25N)
Plasmid#101046PurposeExpresses HA-tagged human Rab11-DN (S25N) in mammalian cellsDepositorAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST MTFR1L-V5
Plasmid#69822PurposeExpresses MTFR1L-V5 in mammalian cellsDepositorInsertMitochondrial fission regulator 1-like (MTFR1L Human)
UseRetroviralTagsv5ExpressionMammalianPromotercmvAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST RBM14-V5
Plasmid#69823PurposeExpresses RBM14-V5 in mammalian cellsDepositorInsertRNA-binding protein 14 (RBM14 Human)
UseRetroviralTagsv5ExpressionMammalianPromotercmvAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastPromoterTEF1pAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
TagsNanoLucExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorInsertAKAP79-AKAR4 (AKAP5 Human)
TagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 myc-Rab10
Plasmid#208366PurposeMammalian expression of myc-tagged human Rab10DepositorAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only