We narrowed to 24,629 results for: Nes

1. pUPD2

   Plasmid

   #68161

   Purpose

   Universal domesticator plasmid for level 0 parts

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK_hygro_BirA

   Plasmid

   #136537

   Purpose

   Lentiviral expression vector for an inducible BirA

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   BirA
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P29

   Plasmid

   #120040

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-6-Ruby-9

   Plasmid

   #197740

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   Ruby reporter tricistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_T7-ARG2

   Plasmid

   #106474

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xHRE_RE:RedF

   Plasmid

   #178314

   Purpose

   HRE RedFirefly luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   HRE RedFirefly reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMaCTag-P21

   Plasmid

   #120032

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   24xSunTag
   

   Use

   Pcr template

   Tags

   24xSunTag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMaCTag-P20

   Plasmid

   #120031

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   10xSuntag
   

   Use

   Pcr template

   Tags

   10xSuntag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLIC-SM-Myc

   Plasmid

   #111189

   Purpose

   High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster Myc Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available Since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pTRANS_231

    Plasmid

    #91120

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTnos (GB0037)

    Plasmid

    #68188

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pP35S (GB0030)

    Plasmid

    #68163

    Purpose

    Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV 35S promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. UAS:Zebrabow-B

    Plasmid

    #118216

    Purpose

    For broad gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Alex Schier, Albert Pan

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 560HTR

    Plasmid

    #24444

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3/CyclinB1-GFP (28)

    Plasmid

    #39871

    Depositor

    Jonathon Pines

    Article

    Jackman et al Mol Biol Cell. 2002 Mar;13(3):1030-45.

    Insert

    CyclinB1 (CCNB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V5A

    Promoter

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-DeltaFosB

    Plasmid

    #68545

    Purpose

    AAV expression of DeltaFosB

    Depositor

    Eric Nestler

    Article

    Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

    Insert

    DeltaFosB-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant of FosB

    Promoter

    CMV

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pETM6-H10-mCherry

    Plasmid

    #66531

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H10

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H10

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin promoter

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid

    #66707

    Purpose

    expresses a single gRNA and VP64-dCas9-VP64

    Depositor

    Charles Gersbach

    Inserts

    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Expression

    Mutation

    D10A and H840A

    Promoter

    hU6 and hUbC

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMaCTag-P23

    Plasmid

    #120034

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PGK-EGFP-FKBPF36V

    Plasmid

    #199765

    Purpose

    plasmid for targeting dTAG into the AAVS1 locus

    Depositor

    Hannes Lans

    Article

    van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

    Insert

    GFP-dTAG
    

    Use

    Tags

    GFP and dTAG

    Expression

    Mammalian and Worm

    Mutation

    Promoter

    hPGK

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-DeltaJunD

    Plasmid

    #68547

    Purpose

    AAV expression of DeltaJunD

    Depositor

    Eric Nestler

    Article

    Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

    Insert

    DeltaJunD-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant

    Promoter

    CMV

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

23. SPDK3959

    Plasmid

    #199246

    Purpose

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

    Insert

    Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    Promoter

    PEBV

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMaCTag-Z15

    Plasmid

    #120058

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMaCTag-Z08

    Plasmid

    #120051

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMaCTag-Z12

    Plasmid

    #120055

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR R4-vas2-integrase-R3

    Plasmid

    #62299

    Purpose

    phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    phageC31 integrase under control of vasa promoter and terminator
    

    Use

    Tags

    HA and SV40 NLS

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSEVA421-Cas9tr

    Plasmid

    #138709

    Purpose

    Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Insert

    tracrRNA-Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMaCTag-P13

    Plasmid

    #120024

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDEST exorh:mGreenLantern

    Plasmid

    #195988

    Purpose

    Transgenesis backbone

    Depositor

    Nicolas Rohner, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. β3-integrin-CFP

    Plasmid

    #26652

    Depositor

    Jonathan Jones

    Article

    Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

    Insert

    β3-integrin (ITGB3 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pEGFP-C2 Chfr (1014)

    Plasmid

    #61853

    Purpose

    mammalian expresion of Chfr fused to EGFP

    Depositor

    Jonathon Pines

    Article

    Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

    Insert

    Chfr (CHFR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pG108-K - AhpC-Glow

    Plasmid

    #185135

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Inserts

    Glow gene
    Bacteroides thetaiotaomicron ahpC promoter
    

    Use

    Pg106

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMC-1-BeYDV-LS-L-11

    Plasmid

    #197717

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV LSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMC-1-BeYDV-LRS-L-11

    Plasmid

    #197718

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    BeYDV LRSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-ADP-MCS-FLAG

    Plasmid

    #192360

    Purpose

    Adipocyte-specific overexpression of genes

    Depositor

    Yifu Qiu

    Article

    Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

    Type

    Empty backbone

    Use

    AAV

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    Promoter

    adiponectin promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV mouse Dscam

    Plasmid

    #18737

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

    Insert

    Dscam (Dscam Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

38. Nano-lantern/pcDNA3

    Plasmid

    #51970

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTRANS_221

    Plasmid

    #91115

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PEP115E

    Plasmid

    #97401

    Purpose

    binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Golgi target

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge