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We narrowed to 6,530 results for: nls

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Showing: 3521 - 3560 of 6530 results

  1. PX388 Bacteroides coprophilus Cas9

    Plasmid
    #68325
    Purpose
    Bacteroides coprophilus Cas9
    Depositor
    Feng Zhang
    Insert
    BcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68325-plasmid-map-sequence-id-125196

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  2. PX378 Sutterella wadsworthensis Cas9

    Plasmid
    #68315
    Purpose
    Sutterella wadsworthensis Cas9
    Depositor
    Feng Zhang
    Insert
    SwCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68315-plasmid-map-sequence-id-119526

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  3. PX387 Mycoplasma mobile Cas9

    Plasmid
    #68324
    Purpose
    Mycoplasma mobile Cas9
    Depositor
    Feng Zhang
    Insert
    MmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68324-plasmid-map-sequence-id-119539

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  4. PX392 Azospirillum B510 Cas9

    Plasmid
    #68329
    Purpose
    Azospirillum B510 Cas9
    Depositor
    Feng Zhang
    Insert
    AzoCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68329-plasmid-map-sequence-id-119551

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  5. Cas9-ryt

    Plasmid
    #246021
    Purpose
    Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex
    Depositor
    Max Scott
    Inserts
    Cas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    246021-plasmid-map-sequence-id-498404

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  6. pICSL50059

    Plasmid
    #245614
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag 2xBP NLS (2xGKRTADGSEFEPKKKRKV)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245614-plasmid-map-sequence-id-495524

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  7. pGGG-TaBSS2-tdTom

    Plasmid
    #242018
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    242018-plasmid-map-sequence-id-492286

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  8. pGGG-TaBSS1-tdTom

    Plasmid
    #242014
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242014-plasmid-map-sequence-id-487879

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  9. SGEP-sh-Hs-PHGDH-67

    Plasmid
    #188671
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188671-plasmid-map-sequence-id-375112

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  21. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Promoter
    SFFV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

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  22. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  24. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  25. pvPE-V4

    Plasmid
    #240288
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240288-plasmid-map-sequence-id-488805

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  26. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid
    #188902
    Purpose
    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9-KRAB(Kox1)-MeCP2
    Use
    Lentiviral
    Tags
    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…
    Promoter
    SFFV
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188902-plasmid-map-sequence-id-372712

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  27. pvPE-V4-Puro

    Plasmid
    #240290
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4-Puro
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240290-plasmid-map-sequence-id-488804

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  29. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220611-plasmid-map-sequence-id-441218

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  32. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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  35. pLH_Scr15

    Plasmid
    #100537
    Purpose
    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    N-terminal version of PhyB (AA 1-621)/ Mutation o…
    Promoter
    FBA1 and TDH3
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100537-plasmid-map-sequence-id-194456

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  38. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

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  39. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

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  40. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

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Showing: 3521 - 3560 of 6530 results