We narrowed to 18,759 results for: nls
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Plasmid#161816PurposeExpresses ABE-P48R in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTetOne-DsRed-cNLS
Plasmid#138528PurposeInducible expression of DsRed-Express2 with nuclear localization signalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDsRed-Express2
Tags3x SV40 cNLSExpressionMammalianPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-PCNA-19-SV40NLS-4
Plasmid#57994PurposeLocalization: Nucleus, Excitation: 555, Emission: 584DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCNA-19 SV40NLS (PCNA Human)
TagsmTagRFPExpressionMammalianPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-PCNA-19-SV40NLS-4
Plasmid#55250PurposeLocalization: Nucleus, Excitation: 383, Emission: 448DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCNA-19 SV40NLS (PCNA Human)
Tags3xSV40 NLS and EBFP2ExpressionMammalianPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-hA3D-BE3
Plasmid#113413PurposeExpresses hA3D-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA
Plasmid#108729PurposeExpresses N-terminus to aa270 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa270
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP
Plasmid#228359PurposeRfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdownDepositorInsertRfxCas13d
UseLentiviralTags2A-EGFP, 2A-mCD4, HA, and NLSExpressionMammalianPromoterEF1AAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS
Plasmid#244833PurposeBacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpyCas9 (WT)
UseCRISPRTags2xSV40 NLS and 10xHis-MBPExpressionBacterialPromoterT7Available sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP
Plasmid#162233PurposeLentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cellsDepositorInsertanti-GFP nanobody lag16lag2 and IFP
UseLentiviralExpressionMammalianAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS-nlstdTomato
Plasmid#132985Purposefull length of nuclear localized tdTomato in pCS expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdTomato
TagsNLS and mycExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PET-WT SpCas9-NLS-6xHis
Plasmid#207373PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertWT SpCas9
UseCRISPRTags6xHisExpressionBacterialPromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-MCS-NLS-3XFLAG-V5
Plasmid#196383PurposeEmpty backbone vector to insert cDNA for strong expression in mammalian cellsDepositorTypeEmpty backboneTags3xFLAG and V5ExpressionBacterial and MammalianPromoterCAGGsAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA
Plasmid#108745PurposeExpresses N-terminus to aa270 of Cre recombinase fused to ABI CIDDepositorInsertCre recombinase fragment N-terminus to aa270
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA
Plasmid#108730PurposeExpresses aa271 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pPFK300_Cas9
Plasmid#104912PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterPhyper spankAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P4:gs:nls:CIBN
Plasmid#135986PurposeExpresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP4 coiled-coil fused to the CIBN domain
ExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS
Plasmid#128833PurposeKnock-in of nuclear EYFP into mouse Elf5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNuclear EYFP into Elf5 locus (Elf5 Mouse)
UseMouse TargetingAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-mA3-BE3
Plasmid#113419PurposeExpresses mA3-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
459 p3E-2A-nlsEGFPpA
Plasmid#195969PurposeGateway p3E 2A fluorophoreDepositorInsert2A-nlsEGFP (nuclear)
UseOtherAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-PCNA-19-SV40NLS-4
Plasmid#55386PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCNA-19 SV40NLS (PCNA Human)
Tags3xSV40 NLS and mCeruleanExpressionMammalianPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV2-CAG-3xNLS-vsfGFP-0
Plasmid#191097PurposeTo express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-vsfGFP-0
UseAAVTagsGreen fluorescent protein bound to enhancer nanob…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS
Plasmid#217478PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1sDepositorInsertNier1s
Tags3xNLS, P2A-EGFP-3xNLSExpressionMammalianPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS
Plasmid#217479PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1bDepositorInsertNier1b
Tags3xNLS, P2A-EGFP-3xNLSExpressionMammalianPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEBFP2-NLS
Plasmid#176682PurposeExpression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEBFP2
ExpressionInsectPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-2xMCP-Ezh2
Plasmid#126590PurposeExpresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backboneDepositorInsert2XMCP-Ezh2 (Ezh2 Synthetic, Mouse)
UseLentiviralTagsNLS-HAExpressionMammalianPromoterhuman ubiquitin C promoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1-NLS-Q2m
Plasmid#129319PurposeQUEEN ATP sensor for 25degC, nucleusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertQUEEN-2m
ExpressionMammalianAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-NLS
Plasmid#103820PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPHR-EYFP-NLS (CRY2 Mustard Weed)
ExpressionMammalianPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pLAT1_Cas9
Plasmid#104908PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA
Plasmid#108746PurposeExpresses aa271 to C-terminus of Cre recombinase fused to PYL CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E5-dTom-nlsdTom
Plasmid#135640PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E5 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40
Plasmid#100030PurposeAAV expression of ArcLightDDepositorInsertArcLightD
UseAAVTags2A-mCherryExpressionMammalianPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2
Plasmid#88915PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertCas9
UseCRISPRExpressionBacterialAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p3E-nlsGFP no-pA
Plasmid#80815PurposenlsGFP without pADepositorInsertp3E-CMV-nlsGFP no pA
ExpressionMammalianAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-CMVmin:nlsGFP pA
Plasmid#80821PurposeCMVmin-promoted nlsGFP with pADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp3E-CMV-nlsGFP pA
ExpressionMammalianPromoterCMVminAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BG4-NLS-mCer
Plasmid#211448Purposemammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequenceDepositorInsertAnti-DNA G-quadruplex single chain antibody BG4
TagsNLS-mCeruleanExpressionMammalianAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BG4-NLS-mVen
Plasmid#211449Purposemammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequenceDepositorInsertAnti-DNA G-quadruplex single chain antibody BG4
TagsNLS-mVenusExpressionMammalianAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-B-actin
Plasmid#117812PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyTagsNLSAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-B-actin
Plasmid#117806PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyTagsNLSAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713-NLS-VP16
Plasmid#103823PurposeSoluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illuminationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPHR-iRFP713-VP16 (CRY2 Mustard Weed)
ExpressionMammalianMutationVP16: G126T (silent, G42G)PromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by