We narrowed to 11,739 results for: 002

1. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

   Plasmid

   #231549

   Purpose

   Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   msfGFP (intramolecular)

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVtrunc msfGFPΔC10-beta-actin

   Plasmid

   #231548

   Purpose

   Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBRPyCAG-Mettl3-dsRed-IRES-puro

   Plasmid

   #61516

   Purpose

   Constitutive expression of murine wild-type Mettl3

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   Mettl3 (Mettl3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHR-mCherry-mHP1alpha

   Plasmid

   #181883

   Purpose

   Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-mHP1alpha (Cbx5 Mustard Weed, Mouse)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPHR-mCherry-mHP1alpha-I163A

   Plasmid

   #181889

   Purpose

   Soluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers,

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-mHP1alpha-I163A (Cbx5 Mustard Weed, Mouse)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   I163A

   Promoter

   CMV

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHR-mCherry-FUSN-mHP1alpha

   Plasmid

   #181892

   Purpose

   Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mustard Weed, Mouse)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6sgp53-mTSG-GFAPCre

   Plasmid

   #100276

   Purpose

   AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Insert

   GFAP promoter (Gfap, GFAP Human, Mouse)
   

   Use

   AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVtrunc msfGFPΔC10-Utr28-222

   Plasmid

   #231558

   Purpose

   Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Utrophin calponin homology domain (UTRN Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSpCas9-hGeminin

   Plasmid

   #199344

   Purpose

   modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin

   Depositor

   Iain Hagan

   Article

   Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1

   Insert

   ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
   

   Use

   Tags

   cas9 c-term fused to hgemenin 1-110 fragment

   Expression

   Mammalian

   Mutation

   cas9 c-term fused to hgemenin 1-110 fragment

   Promoter

   cbh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid

    #210021

    Purpose

    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Human, Synthetic)
    

    Use

    Tags

    MycTag, CTag

    Expression

    Mammalian

    Mutation

    Transferrin receptor transmembrane domain with Y2…

    Promoter

    CMV promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-NOTCH1(Trunc3)-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #157002

    Purpose

    Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    NOTCH1 (NOTCH1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro

    Plasmid

    #192002

    Purpose

    Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-Isoform2 (LYSET Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVTRE3G

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPHR-mCherry-PGL-1-mHP1alpha

    Plasmid

    #181894

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPHR-mCherry-nE-mHP1alpha

    Plasmid

    #181890

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_SERPINB5_WT_V5

    Plasmid

    #83002

    Purpose

    Gateway Donor vector containing SERPINB5, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    SERPINB5 (SERPINB5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S176P; AA190-192delTDTins25;AA194-196delPVQinsSIP…

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AiP12655 - pAAV-AiE0732h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2655)

    Plasmid

    #224002

    Purpose

    AiE0732h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF806-shABC2

    Plasmid

    #186714

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCF806-shABC1

    Plasmid

    #186713

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid

    #181893

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid

    #181891

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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