We narrowed to 23,988 results for: Nes

1. pcDNA5/FRT/TO_NES_HaloTag7

   Plasmid

   #175527

   Purpose

   Strict cytosolic expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   NES-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3xflag-APEX2-NES

   Plasmid

   #164623

   Purpose

   Expresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   NES
   

   Use

   Tags

   3xFLAG and APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSBbi_RP_MicroID2-FLAG-NES

   Plasmid

   #188525

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-p53 (NES-)

   Plasmid

   #12092

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   p53 (NES-) (TP53 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L348A L350A Localized to nucleus.

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-AktAR2-NES

   Plasmid

   #175011

   Purpose

   Cytosol-targeted AktAR2.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   AktAR-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVITRO1-mCherry-NES

   Plasmid

   #186349

   Purpose

   Cytosolic mCherry expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCherry-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti-NES-mTagBFP2

   Plasmid

   #216129

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-MAPKKnucelarExportSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EGFP-uTEV1-NES

   Plasmid

   #171006

   Purpose

   HiLITR protease with C-terminal NNES (Cytoplasmic)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-NES
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NES-iRFP-P4Mx1

   Plasmid

   #108144

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):iRFP:L. penomophila SidM(546-647)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NES-EGFP-cPHx2

    Plasmid

    #116854

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem dimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NES-PAmCherry-cPHx3

    Plasmid

    #116861

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    PAmCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-NPM NESM

    Plasmid

    #13282

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L100A, L102A

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-TORCAR-NES

    Plasmid

    #175015

    Purpose

    Cytosol-targeted TORCAR.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    TORCAR-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_N-DYK_MTHFD1-NES

    Plasmid

    #133022

    Purpose

    NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

    Depositor

    Stefan Kubicek

    Article

    Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

    Insert

    MTHFD1 (MTHFD1 Human)
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGG-NES-TurboID

    Plasmid

    #209393

    Purpose

    Transiently expressing TurboID fused with a nuclear export signal (NES) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    NES-3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RANLS-LEHD-BNES

    Plasmid

    #60973

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    Caspase9 substrate sequence (LEHD) is after NLS s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. GFP-NPM NESD

    Plasmid

    #13283

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa94-aa102

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

18. Linked Free NES

    Plasmid

    #182485

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCMV HDM2(NES-)

    Plasmid

    #12087

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    HDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nuclear-export sequence mutant (NES-): L205A, I20…

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

21. RANLS-IETD-BNES

    Plasmid

    #60972

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pGFP-HBx NESM

    Plasmid

    #24932

    Depositor

    Xin Wang

    Article

    Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

    Insert

    HBx
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    L98A,G99A,L100A

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NES-EGFP-cPHx1

    Plasmid

    #116853

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 )
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    Amino acids 169.329

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVITRO1-TagRFP-NES

    Plasmid

    #184449

    Purpose

    Cytosolic TagRFP expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    TagRFP-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Use

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-Cerulean-WW-NES

    Plasmid

    #84629

    Purpose

    Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    Cerulean-WW-NES
    

    Use

    Tags

    6xHis, Cerulean, Nuclear export signal (NES), T7 …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-NES-ZapCY2

    Plasmid

    #59016

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

    Insert

    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pVITRO1-iRFP702-NES

    Plasmid

    #184457

    Purpose

    Cytosolic iRFP702 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    iRFP702-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NES-EGFP-PH-BTKx1

    Plasmid

    #183651

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Human, Frog)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 2-170

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-NES-ZapCmR1.1

    Plasmid

    #59014

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only