We narrowed to 4,234 results for: Gca

1. pAAV-Ef1a-Coff/Fon-GCaMP6M

   Plasmid

   #137121

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-GFAP(long)-FLEX-GCaMP7f

   Plasmid

   #189612

   Purpose

   Cre-dependent GCaMP7f under the GFAP promoter

   Depositor

   Martin Riccomagno

   Article

   Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

   Insert

   GCaMP7f
   

   Use

   AAV and Cre/Lox

   Tags

   GCaMP7f

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3

   Plasmid

   #112007

   Purpose

   For co-expression of GCaMP6s with mRuby3

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   GCaMP6s-P2A-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-Ef1a-Coff/Fon-GCaMP6F

   Plasmid

   #137124

   Purpose

   Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6F
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-Syn-H2B-jGCaMP8m-WPRE

   Plasmid

   #176748

   Purpose

   AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   H2B-jGCaMP8m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC1812_pCR8-gCasRx-CUG (control gRNA)

   Plasmid

   #118645

   Purpose

   Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gCasRx-CUG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-syn-axon-jGCaMP8s-WPRE

   Plasmid

   #186043

   Purpose

   jGCaMP8s expression in axons under the control of the synapsin promoter

   Depositor

   Loren Looger

   Insert

   axon-jGCaMP8s
   

   Use

   AAV

   Tags

   GAP43-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-syn-axon-jGCaMP8m-WPRE

   Plasmid

   #186042

   Purpose

   jGCaMP8m expression in axons under the control of the synapsin promoter

   Depositor

   Loren Looger

   Insert

   axon-jGCaMP8m
   

   Use

   AAV

   Tags

   GAP43-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-syn-LifeAct-jGCaMP8m-WPRE

    Plasmid

    #186039

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8m
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RabV CVS-N2c(deltaG)-GCaMP6f

    Plasmid

    #73466

    Purpose

    Expresses GCaMP6f for calcium imaging

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    GCaMP6f
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-syn-axon-jGCaMP8f-WPRE

    Plasmid

    #186041

    Purpose

    jGCaMP8f expression in axons under the control of the synapsin promoter

    Depositor

    Loren Looger

    Insert

    axon-jGCaMP8f
    

    Use

    AAV

    Tags

    GAP43-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ai95(RCL-GCaMP6f) targeting vector

    Plasmid

    #61579

    Purpose

    Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-LSL-GCaMP6-fast
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-syn-NES-jGCaMP8m-WPRE

    Plasmid

    #186036

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8m
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-syn-NES-jGCaMP8s-WPRE

    Plasmid

    #186037

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8s
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

    Plasmid

    #211491

    Purpose

    conditional expression of GCaMP8s under artificial promoter Adamts2

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    DIO-GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

    Plasmid

    #211493

    Purpose

    conditional expression of GCaMP8s under artificial promoter Baz1a

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    DIO-GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. VV246: CoChR(C108S)-GCaMP6f-ER in fck

    Plasmid

    #58521

    Purpose

    Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    CoChR(C108S)-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 108 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-Syn-H2B-jGCaMP8s-WPRE

    Plasmid

    #176749

    Purpose

    AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    H2B-jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-syn-NES-jGCaMP8f-WPRE

    Plasmid

    #186035

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8f
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid

    #158758

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    nullCoChR-GCaMP6f-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

    Plasmid

    #87917

    Purpose

    Gateway cloning

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgHTT1, sgGFP2, shCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-GFAP-jGCaMP7c variant 1513

    Plasmid

    #173159

    Purpose

    Expresses jGCaMP7c variant 1513 in astrocytes

    Depositor

    Vincent Vialou, Hervé Chneiweiss

    Insert

    jGCaMP7c variant 1513-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GFAP

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV1-EF1α-F-Flex-jGCaMP7b-WPR

    Plasmid

    #171689

    Purpose

    Flp-dependent expression of jGCaMP7b

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

    Insert

    GCaMP7b
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

    Plasmid

    #58522

    Purpose

    Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S)-TS-GCaMP6f-ER
    

    Use

    Lentiviral

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

    Plasmid

    #215281

    Purpose

    Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    U6

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

    Plasmid

    #215282

    Purpose

    Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 control gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hSyn-DIO-GCaMP6f-WPRE

    Plasmid

    #216275

    Purpose

    AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6f

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human Synapsin 1 (hSyn)

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid

    #141237

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    Human elongation factor-1 alpha (EF-1 alpha)

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-ef1a-DIO-eGCaMP(plus)

    Plasmid

    #201149

    Purpose

    AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP+
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFR-mScarletI-antiFRET-P2A-GCaMP3

    Plasmid

    #191460

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    GCaMP3
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFR-mScarletI-antiFRET-P2A-jGCaMP7c

    Plasmid

    #191461

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jGCaMP7c
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-syn-LifeAct-jGCaMP8f-WPRE

    Plasmid

    #186038

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8f
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-syn-LifeAct-jGCaMP8s-WPRE

    Plasmid

    #186040

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8s under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8s
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CaMKII-GCaMP6f-4x128-mAGNET

    Plasmid

    #166023

    Purpose

    Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

    Depositor

    Xue Han

    Article

    Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

    Insert

    GCaMP6f with 4 miR-128 binding sites
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3

    Plasmid

    #112005

    Purpose

    For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV5 and AAV9

    Insert

    axon-GCaMP6s-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAV Cag Flex H2B Gcamp6f reverse

    Plasmid

    #74155

    Purpose

    Calcium Sensor

    Depositor

    Loren Looger

    Insert

    Gcamp6f
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

    Plasmid

    #163047

    Purpose

    GCaMPer calcium sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-Ef1a-Con/Foff 2.0-GCaMP6F

    Plasmid

    #137123

    Purpose

    Intersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-GCaMP6F
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

    Plasmid

    #162379

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162380

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CAG

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104491

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162386

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3

    Plasmid

    #172922

    Purpose

    For enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3

    Depositor

    GENIE Project

    Has Service

    AAV1 and AAV9

    Insert

    axon-jGCaMP8m-P2A-mRuby3
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain (N terminal on insert…

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162377

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE

    Plasmid

    #162382

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CAG

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104495

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

    Plasmid

    #112004

    Purpose

    For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge