Addgene

We narrowed to 57,713 results for: SAP

Showing: 401 - 450 of 57713 results

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pCDNAIIIB rhoA WT

Plasmid

#61631

Purpose

mammalian expression of rhoA

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-Alpha-Actinin-19

Plasmid

#57346

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV5 Flag Jnk1a1(APY)

Plasmid

#15742

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(TPF) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Thr-190Ala

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag Jnk1a1(TPF)

Plasmid

#15741

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(APY) (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Tyr-192Phe

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pPN022

Plasmid

#91668

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIH-Flag Smad3 EPSM

Plasmid

#27026

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

T179V S204A S208A S213A

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
mEos2-Alpha-Actinin-C-14

Plasmid

#57347

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pPN021

Plasmid

#91667

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGex-3x Flag MKK3

Plasmid

#14814

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK3 (MAP2K3 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 Flag Smad3 EPSM A208S

Plasmid

#27112

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T179V S204A S208A S213A; A208S

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk3a1(2nd methione)

Plasmid

#15746

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1-2nd M (MAPK10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 EPSM A213S

Plasmid

#27113

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T179V S204A S208A S213A; A213S

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 EPSM A204S

Plasmid

#27111

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T179V S204A S208A S213A; A204S

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk3a2(2nd methione)

Plasmid

#15747

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2(2nd methione) (MAPK10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A204S

Plasmid

#27108

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A204S

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A208S

Plasmid

#27109

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A208S

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A213S

Plasmid

#27110

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A213S

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM V179T

Plasmid

#27015

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; V179T

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC AAGY

Plasmid

#27039

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; P181A P182A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 AAGY

Plasmid

#27004

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

P181A P182A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pQCXIH-Flag Smad3 AAGY

Plasmid

#27027

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
GFP-JNK1

Plasmid

#86830

Purpose

mammalian expression of GFP-JNK1

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK1 (MAPK8 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf6 DN T27N

Plasmid

#10831

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 T27N (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T27N. Defective in GTP binding. Dominant negati…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag p38 beta2

Plasmid

#20355

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 beta2 (MAPK11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf1 ActQ71L

Plasmid

#10832

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 Q71L (ARF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q71L. Defective in GTP hydrolysis. Constitutive…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf1 DN T31N

Plasmid

#10833

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 T31N (ARF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T31N. Defective in GTP binding. Dominant negati…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-SMAD6

Plasmid

#170700

Purpose

doxycycline-inducible overexpression of SMAD6

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SMAD6 (SMAD6 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-MYOCD

Plasmid

#170696

Purpose

doxycycline-inducible overexpression of MYOCD

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MYOCD (MYOCD Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-TBX20

Plasmid

#170705

Purpose

doxycycline-inducible overexpression of TBX20

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TBX20 (TBX20 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-TRAIL-R2

Plasmid

#61383

Purpose

Expresses TRAIL receptor 2

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 2 (TNFRSF10B Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-p38beta

Plasmid

#86833

Purpose

mammalian expression of GFP-p38beta

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

p38beta (MAPK11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-JNKKTRmRuby2

Plasmid

#59148

Purpose

ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Tags

mRuby2

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Arf6 ActQ67L

Plasmid

#10835

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 Q67L (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q67L. Defective in GTP hydrolysis. Constitutive…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-Flag MKK6(K82A)

Plasmid

#13519

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6(K82A) (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Lys-82Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDFDuet-MKK6-EE

Plasmid

#82718

Purpose

Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK6 (MAP2K6 Human)

Use

Tags

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pENTR-JNKKTRClover

Plasmid

#59139

Purpose

ORF encoding JNK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCl-HA-YAP

Plasmid

#27007

Depositor

Joan Massague

Article

Alarcon et al Cell. 2009 Nov 13. 139(4):757-69.

Insert

YAP (YAP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only
tetO-TCF21

Plasmid

#170706

Purpose

doxycycline-inducible overexpression of TCF21

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TCF21 (TCF21 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-Puro-TNNT2-Neo

Plasmid

#214013

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRc/RSV Flag MKK3(glu)

Plasmid

#14670

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK3(glu) (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-189Glu Thr-193Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag MKK4(glu)

Plasmid

#14813

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(glu) (MAP2K4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Glu Thr-224Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204342

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-PBX1

Plasmid

#170699

Purpose

doxycycline-inducible overexpression of PBX1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

PBX1 (PBX1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRc/RSV Flag MKK3(ala)

Plasmid

#14669

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK3(ala) (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-189Ala Thr-193Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB cdc42 QL

Plasmid

#61636

Purpose

mammalian expression of cdc42 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Cdc42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-Actin-7

Plasmid

#57339

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Actin (ACTB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 401 - 450 of 57713 results

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