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pHis-TEV-Nsp15
Plasmid#187656PurposeE. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tagDepositorInsertNsp15 (N Severe acute respiratory syndrome coronavirus 2)
UseTagsTEV protease cleavable His6-tagExpressionBacterialMutationnonePromoterT7lacAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEZYeGFP-SARS-CoV-2E
Plasmid#224580PurposeTo express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cellsDepositorInsertSARS-CoV-2E (E SARS-CoV-2)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEZYeGFP-SARS-CoV-2E GGG
Plasmid#224582PurposeTo express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cellsDepositorInsertSARS-CoV-2E GGG (E SARS-CoV-2)
UseTagsGFPExpressionMammalianMutationPBM (LLV) mutated to GGGPromoterCMVAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEZYeGFP-SARS-CoV-2E stop
Plasmid#224581PurposeTo express a GFP tagged version of the Envelope protein of SARS-CoV-2 lacking its PBM in mammalian cellsDepositorInsertSARS-CoV-2E stop (E SARS-CoV-2)
UseTagsGFPExpressionMammalianMutationstop codon before PBMPromoterCMVAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Centrin2 (Nigg UK185)
Plasmid#41147DepositorInsertCentrin-2 (CETN2 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
p35S::IRE1a
Plasmid#135232Purposeconstitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)DepositorInsertIRE1a (IRE1A Mustard Weed)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGT9569
Plasmid#219431PurposeTranscriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOSDepositorInsertLB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsPapE-4LF2ExpressionPlantMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
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SL535
Plasmid#49941PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD RH-2 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-WNT3A
Plasmid#108344Purposesynchronize trafficking of WNT3A from the ER (RUSH System)DepositorInsertWNT3A, Wnt family member 3A (WNT3A Human)
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-WNT3A
Plasmid#108345Purposesynchronize trafficking of WNT3A from the ER (RUSH System)DepositorInsertWNT3A, Wnt family member 3A (WNT3A Human)
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
f2H ∂HD (272)
Plasmid#24428DepositorInsertSULF2 (SULF2 Human)
UseTagsFLAG, His, and MycExpressionMammalianMutationAA 418-716 deleted, 23AA N-terminal truncationPromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-wdb
Plasmid#195069PurposeExpresses FLAG-tagged Drosophila wdb proteinDepositorInsertwiderborst (wdb Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SE
Plasmid#52144PurposeEncodes module 2 with amino acids 12S-16E for recognition of nt 7GDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 2PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2CQ
Plasmid#52143PurposeEncodes module 2 with amino acids 12C-16Q for recognition of nt 7ADepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in Module 2PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SR
Plasmid#52146PurposeEncodes module 2 with amino acids 12S-16R for recognition of nt 7CDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S and 16Q to 16R in Module 2PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only
