We narrowed to 18,603 results for: Tec

1. pRSET-mSA-EGFP

   Plasmid

   #39862

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin fused to EGFP
   

   Use

   Tags

   6xHis, EGFP, and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX851

   Plasmid

   #62883

   Purpose

   N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PX853

   Plasmid

   #62885

   Purpose

   N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-573)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMLM802

   Plasmid

   #27203

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLM800

   Plasmid

   #27202

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXL-CAG-ICeuI-sHRPaNRX3beta

   Plasmid

   #79910

   Purpose

   piggyBac transposon vector, sHRPaNRX3beta

   Depositor

   Joshua Sanes

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPaNRX3beta
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FB430

   Plasmid

   #203621

   Purpose

   TU for the expression of phleomycin resistance.

   Depositor

   Jose Marcos

   Article

   Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

   Insert

   PpcbC:ble:TamdS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FB431

   Plasmid

   #203622

   Purpose

   TU for the expression of terbinafine resistance.

   Depositor

   Jose Marcos

   Article

   Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

   Insert

   PgpdA:ergA:TamdS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX856

   Plasmid

   #62888

   Purpose

   C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS, NLS SV40, and VP64

   Expression

   Mammalian

   Mutation

   Asparagine 863 to Alanine (N863A)

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 Ras-LOCKR-S

    Plasmid

    #216496

    Purpose

    FRET-based reporter for Ras activity, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b - PA

    Plasmid

    #84863

    Purpose

    bacterial expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Tags

    pelB signal sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL715

    Plasmid

    #140385

    Purpose

    Expresses MphR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MphR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJBL723

    Plasmid

    #140393

    Purpose

    Expresses HucR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-HucR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX852

    Plasmid

    #62884

    Purpose

    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid

    #195854

    Purpose

    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    6xHis-AviTag-ALFAtag-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJBL713

    Plasmid

    #140383

    Purpose

    Expresses CtcS protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CtcS-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPLP1H-mCf

    Plasmid

    #229984

    Purpose

    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPLP2H-mCf

    Plasmid

    #229985

    Purpose

    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge