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Showing: 4451 - 4500 of 60949 results

  3. pLX304-EZH1-dPSET

    Plasmid
    #203586
    Purpose
    Expresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.
    Depositor
    Abhishek Chakraborty, William Kaelin
    Article
    Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5
    Insert
    EZH1 (EZH1 Human)
    Use
    Lentiviral
    Tags
    V5-tagged
    Expression
    Mammalian
    Mutation
    deletion of aa 744-747 and partial resistance to …
    Promoter
    CMV
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    203586-plasmid-map-sequence-id-423597

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  6. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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  8. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  10. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid
    #178320
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi
    Inserts
    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178320-plasmid-map-sequence-id-363290

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  11. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179905-plasmid-map-sequence-id-351283

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  12. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  15. pAAV-hsynapsin-DIO-adra2a-shRNA-mCherry

    Plasmid
    #245063
    Purpose
    AAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.
    Depositor
    William Wisden
    Article
    Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;12
    Insert
    AAV-human synapsin promoter-DIO-adra2a-shRNA-mCherry (Adra2a Mouse, Synthetic)
    Use
    AAV, Cre/Lox, and RNAi
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    245063-plasmid-map-sequence-id-489355

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  16. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184106-plasmid-map-sequence-id-364015

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  17. AiP12039 - pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2039)

    Plasmid
    #208401
    Purpose
    AiE0354h is an enhancer sequence designed to drive AAV-mediated transgene expression in Vip+ cortical interneurons
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Lee et al Science. 2023 Oct 13;382(6667):eadf6484.
    Insert
    SYFP2
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    eHGT_354h (enhancer)
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208401-plasmid-map-sequence-id-416929

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  38. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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  39. pTS_ABS

    Plasmid
    #163840
    Purpose
    iPTG inducible expression of abietadiene synthase and geranylgeranyl diphosphate synthase
    Depositor
    Jerome Fox
    Insert
    abietadiene synthase
    Expression
    Bacterial
    Promoter
    Trc
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163840-plasmid-map-sequence-id-339140

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  40. pMF440

    Plasmid
    #62550
    Purpose
    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
    Depositor
    Michael Franklin
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    62550-plasmid-map-sequence-id-397042

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  41. pBac[3xP3 g Tc' v]

    Plasmid
    #86446
    Purpose
    pBAc transposon with transformation marker for Tribolium transgenesis; Rescues vermilion white eyed mutant to black eyes; (transformation plasmid)
    Depositor
    Gregor Bucher
    Insert
    3xP3 g Tc' v
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86446-plasmid-map-sequence-id-171065

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  42. pHERD30T-TnV1acrVIA1

    Plasmid
    #250983
    Purpose
    pHERD30T vector providing the transposon donor with acrVIA1
    Depositor
    Joseph Bondy-Denomy
    Insert
    Transposon donor V1 encoding acrVIA1
    Expression
    Bacterial
    Available Since
    March 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    250983-plasmid-map-sequence-id-500789

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  43. pHERD20T-V3TnAcrVIA1

    Plasmid
    #250984
    Purpose
    pHERD20T vector providing the transposon donor with acrVIA1
    Depositor
    Joseph Bondy-Denomy
    Insert
    Transposon donor V3 encoding acrVIA1
    Expression
    Bacterial
    Available Since
    March 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    250984-plasmid-map-sequence-id-500738

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  44. pCV020-tetQ

    Plasmid
    #232826
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - tetracycline
    Depositor
    Athanasios Typas
    Insert
    tetQ
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232826-plasmid-map-sequence-id-462958

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  45. pCV019-catP

    Plasmid
    #232825
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - chloramphenicol
    Depositor
    Athanasios Typas
    Insert
    catP
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232825-plasmid-map-sequence-id-462956

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  46. tdTurboRFP-VSVG-16

    Plasmid
    #58066
    Purpose
    Localization: Golgi/ER Transport, Excitation: 553, Emission: 576
    Depositor
    Michael Davidson
    Insert
    VSVG
    Tags
    tdTurboRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. PB-CMV-MCS-Puro

    Plasmid
    #219794
    Purpose
    Mammalian expression plasmids using piggyBac Transposon System
    Depositor
    Keiichi Nakayama
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219794-plasmid-map-sequence-id-441249

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  48. pCV006-ermg

    Plasmid
    #232824
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - erythromycin
    Depositor
    Athanasios Typas
    Insert
    ermG
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232824-plasmid-map-sequence-id-462957

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  49. tdTomato-Lysosomes-20

    Plasmid
    #58109
    Purpose
    Localization: Lysosome Membrane, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 4451 - 4500 of 60949 results