4,202 results

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Tobacco etch virus (TEV) protease

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27

Plasmid

#22902

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K27 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3/er-(n2)oxStayGold(c4)v2.0

Plasmid

#186296

Purpose

A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)v2.0

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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mTLR4

Plasmid

#13085

Depositor

Ruslan Medzhitov

Insert

TLR4 (Tlr4 Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pAG241 FKBP-CFAST10

Plasmid

#130814

Purpose

Expresses FKBP-CFAST10 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST10

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ153

Plasmid

#69304

Purpose

Gateway entry vector with pco-dCas9-3X(SRDX)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-3X(SRDX) (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

SRDX (X3) repressor fusion; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-neo

Plasmid

#32425

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

GFP-T2A-neo

Expression

Insect

Promoter

Actin5c

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pHEE401E

Plasmid

#71287

Purpose

Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Inserts

gRNA scaffold
zCas9

Use

CRISPR; Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pGEM-Oct4

Plasmid

#16352

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Expression

Bacterial

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEF1a-FB-dCas9-puro

Plasmid

#100547

Purpose

Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Inserts

N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin

Use

CRISPR

Tags

FLAG, Biotin-acceptor-site (Avitag)

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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PGK-H2BmCherry

Plasmid

#21217

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-mCherry

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Article

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Insert

NMHC II-C (Myh14 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Myc3-Nrf2

Plasmid

#21555

Purpose

Mammalian expression of human Nrf2 with Myc tag

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

myc3

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP46

Plasmid

#22584

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP46 (USP46 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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cyto-Ruby3-iATPSnFR1.0

Plasmid

#102551

Purpose

expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

Ruby3-iATPSnFR1.0

Tags

Ruby3

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-mTH-GFP

Plasmid

#99128

Purpose

An AAV genome that expresses GFP from the mouse TH promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

mTH

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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