We narrowed to 22,783 results for: Sis

1. pHyg-AID*

   Plasmid

   #99515

   Purpose

   C-terminal AID* degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-114) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Mutation

   internal inversion of nucleotides 265-312 of the …

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRosa26-GT

   Plasmid

   #40025

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   beta-globin intron
   Neo
   tdT-3Myc
   diphteria toxin A
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZLblasti-HA-ERK5

   Plasmid

   #53175

   Purpose

   Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK5 (MAPK7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pKan-PRFA1-9myc-AID*(N)

   Plasmid

   #99528

   Purpose

   N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-117) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Promoter

   RFA1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKan-PCUP1-9myc-AID*(N)

   Plasmid

   #99527

   Purpose

   N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-117) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Promoter

   CUP1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBEC8

   Plasmid

   #187599

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

   Plasmid

   #211904

   Purpose

   Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   CD19-CAR-28z-2A-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hPGK1

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Myc-CYLD-C601A

   Plasmid

   #60027

   Purpose

   Myc-tagged CYLD catalytic inactive (C601A) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   CMV

   Available Since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE80L-SH3

   Plasmid

   #160331

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   SH3
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid

    #82613

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

    Insert

    3xFLAG-dCas9
    

    Use

    Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSNV2 CHER NLS cop1at(335-675)

    Plasmid

    #44972

    Depositor

    Thanos Halazonetis

    Article

    Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

    Insert

    mCherry-NLS-COP1 (COP1 Mustard Weed)
    

    Tags

    NLS and mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-334

    Promoter

    pCMV IE

    Available Since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-mCherry-SPASTIN

    Plasmid

    #180647

    Purpose

    Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, has siRNA resistance to GAACAGUGUG…

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NPY-mApple

    Plasmid

    #83498

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRosa26-TG

    Plasmid

    #40026

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (ATG)
    beta-globin intron
    Neo
    GFP
    diphteria toxin A
    

    Expression

    Mammalian

    Mutation

    deleted nucleotides 1-274 and insertion of FRT, …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJY-RpABE-PDS3_IMS

    Plasmid

    #112875

    Purpose

    binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

    Depositor

    Jae-Young Yun, Jin-Soo Kim

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting PDS3
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-alpha(740-977)-GFP

    Plasmid

    #100731

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2A1 (Ap2a1 Mouse)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-C1-FAK-HA-V744G

    Plasmid

    #35040

    Depositor

    Anna Huttenlocher

    Article

    Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

    Insert

    focal adhesion kinase (Ptk2 Mouse)
    

    Tags

    2x HA and mCherry

    Expression

    Mammalian

    Mutation

    GFP-FAK-V744G was generated from GFP-FAK using th…

    Available Since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid

    #84730

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7C-DNAJB6

    Plasmid

    #177657

    Purpose

    Recombinant protein synthesis of human DNAJB6

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJB6 (DNAJB6 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-Htr5b(3.7)-EGFP-sv40

    Plasmid

    #184411

    Purpose

    AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Mouse Htr5b gene

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUB-nSypHer (NLS:sypHer:NLS)

    Plasmid

    #84731

    Purpose

    Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    nSypHer
    

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7C-DNAJB4

    Plasmid

    #177656

    Purpose

    Recombinant protein synthesis of human DNAJB4

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pT/Caggs-NRAS G12V-IRES-ASK1

    Plasmid

    #221074

    Purpose

    Expresses NRAS G12V and ASK1 in mammalian cells

    Depositor

    Hidenori Ichijo

    Article

    Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

    Inserts

    NRAS (NRAS Human)
    ASK1
    

    Expression

    Mammalian

    Mutation

    G12V

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUBQ10:AtFLS2

    Plasmid

    #202186

    Purpose

    Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

    Depositor

    Gitta Coaker

    Article

    Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

    Insert

    Flagellin sensing 2 (FLS2 Mustard Weed)
    

    Tags

    HA

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid

    #49520

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38M-P750-luxAB-DAS+4
    

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SEP-Nanoluc-cyOFP1

    Plasmid

    #141082

    Purpose

    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc-cyOFP1
    

    Use

    Lentiviral

    Mutation

    Deletion of amino acids DISGG from positions 673 …

    Promoter

    EF1α

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCold I-Hero7

    Plasmid

    #187926

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero7

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero7 (SERF2 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCA-T-intron(Neo)-G(LNL)

    Plasmid

    #36908

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Neo
    

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AID-BFP-loxP_myo2_neoR

    Plasmid

    #194054

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Tags

    bfp

    Expression

    Worm

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEM-PH-citrine

    Plasmid

    #131406

    Purpose

    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

    Depositor

    Lars Hufnagel

    Article

    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

    Insert

    PH
    

    Use

    In vitro transcription

    Tags

    citrine yellow fluorescent protein

    Promoter

    T7

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

    Plasmid

    #127365

    Purpose

    Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pZJQIBEBT003

    Plasmid

    #140053

    Purpose

    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

    Depositor

    Jia Zhang

    Article

    Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

    Insert

    eMutS-L157C-G233C-CBM-EGFP
    

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    L157C, G233C

    Promoter

    T7

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. sox10:PDGFRA wild-type

    Plasmid

    #98696

    Purpose

    I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT2Apuro-TRE-synPCB2.0

    Plasmid

    #139481

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tol2 transposon donor vector

    Expression

    Mammalian

    Promoter

    Tet responsive element

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p35S::IRE1a

    Plasmid

    #135232

    Purpose

    constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    IRE1a (IRE1A Mustard Weed)
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

    Plasmid

    #127363

    Purpose

    Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

    Plasmid

    #65662

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. sox10:PDGFRA mutant

    Plasmid

    #98697

    Purpose

    I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Mutation

    exon 8 and 9 deletion

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge