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Plasmid#233446PurposePiggyBac donor plasmid to overexpress ADAR2dd-PCPDepositorInsertADAR2dd-PCP-T2A-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
Plasmid#233441PurposePiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)DepositorInsertCCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP
Plasmid#233444PurposePiggyBac donor plasmid to overexpress ADAR2dd-MCPDepositorInsertADAR2dd-MCP-T2A-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA
Plasmid#233046PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.DepositorInsertRfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA
Plasmid#233045PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.DepositorInsertRfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry
Plasmid#232244PurposeMammalian expression of N581K RLIM-GFP-IRES-mCherryDepositorInsertN581K RLIM-GFP-IRES-mCherry (RLIM Human)
UseTagsAcGFP1ExpressionMammalianMutationN581KPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry
Plasmid#232245PurposeMammalian expression of I77P I78P REX1-GFP-IRES-mCherryDepositorInsertI77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)
UseTagsAcGFP1ExpressionMammalianMutationI77P I78PPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)
Plasmid#225454PurposeExpress mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationT236D H237D K299D N300DPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro
Plasmid#212675PurposeExpress tagged hRb phosphosite mutantDepositorInsertRb (RB1 Human)
UseLentiviralTagsHAExpressionMutation15 CDK phospho-sites are mutated to alanines, and…PromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
Plasmid#179840Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC) and eGFPExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)
Plasmid#221385PurposeLentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance geneDepositorInsertSpCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro
Plasmid#211616PurposeRhno1 Plk1mut7ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmycExpressionMammalianMutationS51A, T52A, S141A, S162A, S163A, S164A, S178APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-Q2L7-T2176C-UGI-Puro
Plasmid#209648PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-Q2L7-T2176C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-Puro
Plasmid#211619PurposeRhno1 delta TOPBP1 (T202A)DepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationT202APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro
Plasmid#208399Purposecodes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistanceDepositorArticleInserthU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
UseCRISPR and LentiviralTagsiRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro
Plasmid#208398Purposecodes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistanceDepositorArticleInserthU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
UseCRISPR and LentiviralTagsE2-CrimsonExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro
Plasmid#208400Purposecodes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistanceDepositorArticleInserthU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
UseCRISPR and LentiviralTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro
Plasmid#209644PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100N
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro
Plasmid#209643PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100C
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro
Plasmid#198851PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND1-W86-R17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro
Plasmid#198861PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND6-W82-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND5-W68-R15-G1333N-UGI-Puro
Plasmid#198859PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND5-W68-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND4-W24-R17-G1333N-UGI-Puro
Plasmid#198857PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND4-W24-R17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND3-Q26-R19-G1397N-UGI-Puro
Plasmid#198855PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertND3-Q26-R19 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND2-W30-R18-G1333N-UGI-Puro
Plasmid#198853PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND2-W30-R18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CYTB-W77-R14-G1333N-UGI-Puro
Plasmid#198849PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCYTB-W77-R14 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX3-W16-R15-G1333N-UGI-Puro
Plasmid#198847PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX3-W16-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX1-W25-R18-G1333N-UGI-Puro
Plasmid#198843PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX1-W25-R18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP6-W68-R17-G1333N-UGI-Puro
Plasmid#198839PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP6-W68-R17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND1-W86-L14-G1333C-UGI-Puro
Plasmid#198850PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND1-W86-L14 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND5-W68-L15-G1333C-UGI-Puro
Plasmid#198858PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND5-W68-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX3-W16-L17-G1333C-UGI-Puro
Plasmid#198846PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX3-W16-L17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND3-Q26-L18-G1397C-UGI-Puro
Plasmid#198854PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertND3-Q26-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro
Plasmid#198838PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP6-W68-L16 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro
Plasmid#198856PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND4-W24-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro
Plasmid#198852PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND2-W30-L16 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro
Plasmid#198848PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCYTB-W77-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro
Plasmid#198842PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertCOX1-W25-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro
Plasmid#198860PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertND6-W82-L15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA
Plasmid#191686PurposeFor knocking p2a-tdTomato into PTPRO locusDepositorInsertPTPRO homology arms and p2a-tdTomato
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only