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We narrowed to 9,416 results for: Coli

Showing: 4501 - 4550 of 9416 results

  1. pOP3BP

    Plasmid
    #112602
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GB1 fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-GB1
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112602-plasmid-map-sequence-id-220216

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  2. Sc PCNA-pET(11a)

    Plasmid
    #239197
    Purpose
    Overexpress Sc PCNA in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PCNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239197-plasmid-map-sequence-id-474410

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  3. pOP3GP

    Plasmid
    #112606
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GST fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Avi - 8His-GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112606-plasmid-map-sequence-id-220235

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  5. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Neil Olszewski
    Type
    Empty backbone
    Use
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84652-plasmid-map-sequence-id-162124

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  6. pENTR L1-13XLexAop2-R5

    Plasmid
    #41433
    Depositor
    Steve Stowers
    Insert
    13XLexAop2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41433-plasmid-map-sequence-id-55242

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  7. pENTR L1-13XLexAop2-L4

    Plasmid
    #41434
    Depositor
    Steve Stowers
    Insert
    13XLexAop2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41434-plasmid-map-sequence-id-55244

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  8. pAIP4_1EDG

    Plasmid
    #234144
    Purpose
    Heterologous protein expression of GUNA_RUMCH in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EDG
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234144-plasmid-map-sequence-id-464110

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  11. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  19. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

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  22. pST_140_LVL2 cam

    Plasmid
    #179334
    Purpose
    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).
    Depositor
    Anke Becker
    Article
    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:
    Insert
    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…
    Promoter
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179334-plasmid-map-sequence-id-357079

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  25. pCCM’

    Plasmid
    #162709
    Purpose
    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    p15A origin replaced with high-copy colE1 origin
    Promoter
    PLtet0-1 promoter
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162709-plasmid-map-sequence-id-341131

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  27. pCB’

    Plasmid
    #162703
    Purpose
    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Use
    Tags
    Expression
    Bacterial
    Mutation
    5513T>G (tetR E37A); 7758G>T (second tet op…
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162703-plasmid-map-sequence-id-324199

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  38. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Johan Paulsson
    Article
    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    127855-plasmid-map-sequence-id-253039

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  44. GFP-itis pBE-t

    Plasmid
    #195342
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195342-plasmid-map-sequence-id-383223

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  46. pMM627

    Plasmid
    #112532
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23107, PL(HrtO), and ProD
    Available Since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112532-plasmid-map-sequence-id-217573

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  48. GFP-itis pBE-nt

    Plasmid
    #195343
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195343-plasmid-map-sequence-id-383225

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Showing: 4501 - 4550 of 9416 results