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Showing: 4501 - 4550 of 9181 results

  6. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid
    #186413
    Purpose
    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    lacZ (lacZ E. coli)
    Use
    Gateway destination vector
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186413-plasmid-map-sequence-id-369044

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  7. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169657-plasmid-map-sequence-id-365632

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  8. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid
    #169659
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169659-plasmid-map-sequence-id-364557

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  10. pCCM’ ΔacRAF

    Plasmid
    #162710
    Purpose
    Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    pCCM' with a deletion of putative rubisco ch…
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162710-plasmid-map-sequence-id-324185

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  11. pBB0238-TEV-His12

    Plasmid
    #137038
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137038-plasmid-map-sequence-id-291947

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  15. pJOG252

    Plasmid
    #80584
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80584-plasmid-map-sequence-id-151870

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  16. pJOG253

    Plasmid
    #80585
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80585-plasmid-map-sequence-id-151872

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  17. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

    Plasmid
    #70195
    Purpose
    Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector
    Depositor
    Richard Murray
    Article
    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.
    Insert
    tetR-deGFP fusion
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    70195-plasmid-map-sequence-id-125453

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  18. R0063 (pLuxR-pR)_EB

    Plasmid
    #66013
    Purpose
    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66013-plasmid-map-sequence-id-114131

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  19. R0063 (pLuxR-pR)_FB

    Plasmid
    #66014
    Purpose
    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66014-plasmid-map-sequence-id-114133

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  20. R0063 (pLuxR-pR)_GB

    Plasmid
    #66015
    Purpose
    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66015-plasmid-map-sequence-id-114135

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  23. pOP5GP

    Plasmid
    #112610
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112610-plasmid-map-sequence-id-220240

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  24. pAIP4_1UXZ

    Plasmid
    #234166
    Purpose
    Heterologous protein expression of cellulase B in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UXZ
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234166-plasmid-map-sequence-id-464132

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  25. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

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  26. pET-Bst-LF-Idaho

    Plasmid
    #170469
    Purpose
    Expresses His-tagged Bst-LF-Idaho in E. coli
    Depositor
    Jonathan Schmid-Burgk
    Insert
    Bst-LF-Idaho
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170469-plasmid-map-sequence-id-338354

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  27. pOP5GT

    Plasmid
    #112611
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112611-plasmid-map-sequence-id-220247

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  28. pOP5MP

    Plasmid
    #112612
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-MBP and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112612-plasmid-map-sequence-id-220250

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  29. pOP5BT

    Plasmid
    #112609
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112609-plasmid-map-sequence-id-220239

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  30. pAIP4_3S60

    Plasmid
    #234231
    Purpose
    Heterologous protein expression of lectin OAA in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3S60
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234231-plasmid-map-sequence-id-464330

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  31. pAIP4_3H6Q

    Plasmid
    #234185
    Purpose
    Heterologous protein expression of macrocypin-1a in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3H6Q
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234185-plasmid-map-sequence-id-464150

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  32. pAIP4_4Q2S

    Plasmid
    #234177
    Purpose
    Heterologous protein expression of C20G4.08 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4Q2S
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234177-plasmid-map-sequence-id-464142

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  33. pAIP4_4O6G

    Plasmid
    #234175
    Purpose
    Heterologous protein expression of uncharacterized protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4O6G
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234175-plasmid-map-sequence-id-464140

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  34. pAIP4_3K1H

    Plasmid
    #234157
    Purpose
    Heterologous protein expression of O25709_HELPY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3K1H
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234157-plasmid-map-sequence-id-464123

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  35. MZB184

    Plasmid
    #228488
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228488-plasmid-map-sequence-id-458447

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  36. MZB223

    Plasmid
    #228491
    Purpose
    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    8xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228491-plasmid-map-sequence-id-455392

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  37. pJBL7042

    Plasmid
    #167213
    Purpose
    overexpression of MerR in E. coli for cell-free mercury sensing
    Depositor
    Julius Lucks
    Insert
    MerR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167213-plasmid-map-sequence-id-330998

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  38. pJBL7043

    Plasmid
    #167214
    Purpose
    overexpression of ArsR in E. coli for cell-free arsenic sensing
    Depositor
    Julius Lucks
    Insert
    ArsR EP3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167214-plasmid-map-sequence-id-331005

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  39. pJBL7047

    Plasmid
    #167217
    Purpose
    overexpression of CadR in E. coli for cell-free cadmium sensing
    Depositor
    Julius Lucks
    Insert
    CadR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167217-plasmid-map-sequence-id-331011

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  40. pOP5TT

    Plasmid
    #112613
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-thioredoxin and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112613-plasmid-map-sequence-id-220251

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  41. pOP5BP

    Plasmid
    #112608
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112608-plasmid-map-sequence-id-220237

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  42. pET28b(+)-TM0848

    Plasmid
    #67287
    Purpose
    Expresses TM0848 in E. coli
    Depositor
    SeWon Suh
    Insert
    GTP-binding protein YqeH
    Use
    Tags
    N tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only

  43. pOpen-ECOpolA

    Plasmid
    #165532
    Purpose
    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    E. coli DNA Polymerase I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165532-plasmid-map-sequence-id-339121

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  44. pExp-CHis

    Plasmid
    #112559
    Purpose
    Used for expression of protein in E. coli as C-terminual 8His fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112559-plasmid-map-sequence-id-220671

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  45. pAIP4_2H7W

    Plasmid
    #234220
    Purpose
    Heterologous protein expression of chagasin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234220-plasmid-map-sequence-id-464185

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  46. pAIP4_2NT3

    Plasmid
    #234208
    Purpose
    Heterologous protein expression of response regulator homolog in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2NT3
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234208-plasmid-map-sequence-id-464173

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  50. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

    Enlarge

Showing: 4501 - 4550 of 9181 results