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We narrowed to 34,413 results for: CaS;

Showing: 1801 - 1820 of 34413 results

  5. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  6. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid
    #176250
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176250-plasmid-map-sequence-id-346579

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  7. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

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  14. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Melissa Little, Sara Howden
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Expression
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

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  18. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155046-plasmid-map-sequence-id-308670

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  20. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid
    #178091
    Purpose
    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178091-plasmid-map-sequence-id-350141

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Showing: 1801 - 1820 of 34413 results